A scanning electron microscope study of the capillary loops of oral epitherial papillae using corrosive resin casts III, palate mucosa

• Published in: Japanese Journal of Oral Biology Vol. 28; no. 3; pp. 360 - 362
• Main Authors: Kishi, Yoshiaki, Wang, Tuncheng, So, Seigyo, Nakamura, Satoshi, Matsuo, Masato, Takahashi, Kazuto
• Format: Journal Article
• Language: Japanese; English
• Published: Japanese Association for Oral Biology 1986
• ISSN: 0385-0137
• DOI: 10.2330/joralbiosci1965.28.360

「Introduction」The three-dimensional characteristic vascular network of oral epithelial papillae has already reported in this journal1, 2), using the technique of injection of the blood vessels, with low-viscosity resin and which were then examined with a scanning electron microscope (SEM). The detailed three-dimensional vascular network of the papillae in palatal mucosa was also observed in this study. 「Materials and Methods」Five adult dogs were anesthetized with sodium pentobarbital and the bilateral common carotid arteries were cannulated. A sufficient amount of Ringer's saline solution was perfused into the arteries until the color of the jugular veins appeared clear. After saline perfusion, 2% glutaraldehyde in phosphate buffer was perfused to fix vessels. After fixation, the other tubes were inserted into the bilateral external calotid artery, into which the collored synthetic resin (Melcox) was injected until the whole palate was perfused with the resin. After polymerization, the specimens were decalcified with 10% nitric acid and the soft tissues were dissolved with 10 % potassium hydroxide.