The cytotoxicity of water-soluble carbon nanotubes on human embryonic kidney and liver cancer cells

• Published in: New carbon materials Vol. 33; no. 1; pp. 36 - 45
• Main Authors: Yu, Shi-ping, Su, Xu-dong, Du, Jing-lei, Wang, Jun-li, Gao, Yu-duan, Zhang, Li, Chen, Lin, Yang, Yong-zhen, Liu, Xu-guang
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2018
• Subjects: Cytotoxicity; Hepatoma cell; Human embryonic kidney cells; Water-soluble carbon nanotubes; Hepatoma cell; Cytotoxicity; Human embryonic kidney cells; Water-soluble carbon nanotubes
• ISSN: 1872-5805; 1872-5805
• DOI: 10.1016/S1872-5805(18)60325-7

The cytotoxicity of polyethylene glycol-modified carbon nanotubes (PEG-CNTs) on human embryonic kidney cells (293T) and hepatoma cells (HepG2) was studied in vitro. The effect of the concentration of PEG-CNTs on the cell activity and survival rate was detected by a colorimetric assay method after exposure for 24, 48 and 72 h. The dead HepG2 cells obtained by incubation in solutions with different concentrations of PEG-CNTs for 24 h can be stained by a dye, propidium iodide, and the cell mortality was determined by flow cytometry. Results show that the toxicity of PEG-CNTs with a good water solubility towards 293T and HepG2 cells increases with increasing PEG-CNT concentration. When the concentration of the PEG-CNTs is less than 100 μg/mL, the toxicity of the PEG-CNTs is Grade I (non-toxic) and when it is greater than 100 μg/mL but less than 200 μg/mL it is Grade II (mildly toxic), according to the ISO2109932-5 cytotoxicity standard. The toxicity grade does not change with increased time.