Metabolizable 111In chelate conjugated anti-idiotype monoclonal antibody for radioimmunodetection of lymphoma in mice

• Published in: European journal of nuclear medicine Vol. 12; no. 9; p. 455
• Main Authors: Haseman, M K, Goodwin, D A, Meares, C F, Kaminski, M S, Wensel, T G, McCall, M J, Levy, R
• Format: Journal Article
• Language: English
• Published: Germany 01.12.1986
• Subjects: Animals; Antibodies, Anti-Idiotypic - immunology; Antibodies, Monoclonal - immunology; Chelating Agents; Edetic Acid - analogs & derivatives; Edetic Acid - metabolism; Ethylenediamines - metabolism; Immunoglobulin Idiotypes - immunology; Indium - metabolism; Liver - metabolism; Lymphoma - diagnostic imaging; Lymphoma - immunology; Lymphoma - metabolism; Metabolic Clearance Rate; Mice; Mice, Inbred C3H; Radioisotopes - metabolism; Radionuclide Imaging; Rats; Spleen - metabolism; Succinimides - metabolism
• ISSN: 0340-6997
• DOI: 10.1007/bf00254750

The relative biological properties of 111In-labeled monoclonal antibodies (MoAb) coupled with a conventional bifunctional chelate (BC) and a new, enzyme metabolizable, bifunctional chelate (BCM) were investigated. A rat IgG2a MoAb against idiotype from a mouse B-cell lymphoma was utilized. Mice bearing B-cell lymphomas in the subcutaneous tissues of the flank were given IV-injections of labeled MoAb and imaged or killed for organ counting at 24 h or 48 h. Rat anti-dinitrophenyl IgG2a MoAb and non-specific polyclonal mouse IgG were used as controls. Compared to BC, the use of BCM resulted in a substantial decrease in blood background activity, a shorter biological half-life and an increase in tumor to blood ratio at the expense of a moderate decrease in absolute tumor uptake. The versatile chemistry of these C-1 substituted bifunctional chelates provides a variety of possible enzyme cleavable moieties for further investigation.