Molecular identification and whole genome sequence analyses of methicillin-resistant and mastitis-associated Staphylococcus aureus sequence types 6 and 2454 isolated from dairy cows
The emergence of antimicrobial-resistant and mastitis-associated is of great concern due to the huge economic losses worldwide. Here, we report draft genome sequences of two strains which were isolated from raw milk samples obtained from mastitis-infected cows in Bangladesh. The strains were isolate...
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Published in | Journal of genomics Vol. 12; pp. 19 - 25 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Australia
Ivyspring International Publisher
2024
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Subjects | |
Online Access | Get full text |
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Summary: | The emergence of antimicrobial-resistant and mastitis-associated
is of great concern due to the huge economic losses worldwide. Here, we report draft genome sequences of two
strains which were isolated from raw milk samples obtained from mastitis-infected cows in Bangladesh. The strains were isolated and identified using conventional microbiological and molecular polymerase chain reaction (PCR) methods. Antibiotic susceptibility testing was performed. Genomic DNA of the two strains was extracted and the strains were sequenced using the Illumina NextSeq 550 platform. The assembled contigs were analyzed for virulence determinants, antimicrobial resistance genes, extra-chromosomal plasmids, and multi-locus sequence type (MLST). The genomes of the two strains were compared with other publicly available genome sequences of
strains. The raw read sequences were downloaded and all sequence files were analyzed identically to generate core genome phylogenetic trees. The genome of BR-MHR281strain did not harbour any antibiotic resistance determinants, however BR-MHR220 strain harbored
and
genes. Analysis of BR-MHR220 strain revealed that it was assigned to sequence type (ST-6), clonal complex (CC) 5 and
type t304, while BR-MHR281 strain belonged to ST-2454, CC8, and harbored the
type t7867. The findings of the present study and the genome sequences of BR-MHR220 and BR-MHR281 strains will provide data on the detection and genomic analysis and characterization of mastitis-associated
in Bangladesh. In addition, the findings of the present study will serve as reference genomes for future molecular epidemiological studies and will provide significant data which help understand the prevalence, pathogenesis and antimicrobial resistance of mastitis-associated
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interest exists. Mohammad H. Rahman and Mohamed E. El Zowalaty are equal first authors and were arranged by order of increasing seniority. |
ISSN: | 1839-9940 1839-9940 |
DOI: | 10.7150/jgen.90833 |