Anti-Helicobacter pylori effect of CaG-NANA, a new sialic acid derivative

AIM To investigate the bactericidal effects of calcium chelated N-acetylneuraminic acid-glycomacropeptide (CaG-NANA) against Helicobacter pylori (H. pylori ).METHODS For manufacture of CaG-NANA, calcium (Ca) was combined with glycomacropeptide (GMP) by chelating,and N-acetylneuraminic acid (NANA) wa...

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Published inWorld journal of gastrointestinal pathophysiology Vol. 7; no. 4; pp. 300 - 306
Main Authors Rhee, Yun-Hee, Ku, Hyun-Jeong, Noh, Hye-Ji, Cho, Hyang-Hyun, Kim, Hee-Kyong, Ahn, Jin-Chul
Format Journal Article
LanguageEnglish
Published United States Baishideng Publishing Group Inc 15.11.2016
Subjects
acid-glycomacropeptide
Basic Study
Calcium
chealated
Helicobacter
N-acetylneuraminic
pylori
Calcium chealated N-acetylneuraminic acid-glycomacropeptide
Helicobacter pylori
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Summary:AIM To investigate the bactericidal effects of calcium chelated N-acetylneuraminic acid-glycomacropeptide (CaG-NANA) against Helicobacter pylori (H. pylori ).METHODS For manufacture of CaG-NANA, calcium (Ca) was combined with glycomacropeptide (GMP) by chelating,and N-acetylneuraminic acid (NANA) was produced with Ca-GMP substrate by an enzymatic method. The final concentration of each component was 5% Ca,7% NANA, 85% GMP, and 3% water. For in vitro study,various concentrations of CaG-NANA were investigated under the minimal inhibitory concentration (MIC). For in vivo study, CaG-NANA was administered orally for 3 wk after H. pylori infection. The levels of inflammatory cytokines in blood were analyzed by enzyme-linked immunosorbent assay and eradication of H. pylori was assessed by histological observation.RESULTS The time-kill curves showed a persistent decrease in cell numbers, which depended on the dose of CaG-NANA,and MIC of CaG-NANA against H. pylori was 0.5% in vitro . Histopathologic observation revealed no obvious inflammation or pathologic changes in the gastric mucosa in the CaG-NANA treatment group in vivo . The colonization of H. pylori was reduced after CaG-NANA treatment. The levels of interleukin (IL)-6, IL-1β, tumor necrosis factor-α, and IL-10 were also decreased by CaG-NANA. CONCLUSION CaG-NANA demonstrates effective anti-bactericidal activity against H. pylori both in vitro and in vivo .
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Author contributions: Rhee YH performed the majority of experiments, analyzed the data, and wrote the manuscript; Ku HJ and Noh HJ participated in animal experiments; Cho HH, Kim HK and Ahn JC designed and coordinated the research.
Correspondence to: Jin-Chul Ahn, MD, Department of Biomedical Science, College of Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 31116, South Korea. jcahn@dankook.ac.kr
Telephone: +82-1899-3700
ISSN:2150-5330
2150-5330
DOI:10.4291/wjgp.v7.i4.300