Inhibited phospholipid translocation across interdigitated phosphatidylglycerol vesicle membranes

This study evaluates the ability of atropine, a well-known anticholinergic agent, to alter the rate of phospholipid translocation or ‘flip-flop’ across phosphatidylglycerol vesicle membranes. Atropine does not effect the inward translocation of PG-NBD, a fluorescently labeled phosphatidylglycerol pr...

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Bibliographic Details
Published inJournal of supramolecular chemistry Vol. 1; no. 1; pp. 17 - 21
Main Authors Boon, J.Middleton, McClain, Robert L, Breen, J.J, Smith, Bradley D
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 2001
Subjects
atomic force microscopy
bilayer membrane
flip-flop
fluorescence spectrocopy
interdigitated membrane
interdigitated membrane
atomic force microscopy
flip-flop
bilayer membrane
fluorescence spectrocopy
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Summary:This study evaluates the ability of atropine, a well-known anticholinergic agent, to alter the rate of phospholipid translocation or ‘flip-flop’ across phosphatidylglycerol vesicle membranes. Atropine does not effect the inward translocation of PG-NBD, a fluorescently labeled phosphatidylglycerol probe, across fluid-phase 1-palmitoyl-2-oleoyl-phosphatidylglycerol (POPG) membranes. A combination of fluorescence methods and atomic force microscopy was used to show that atropine induces dipalmitoylphosphatidylglycerol (DPPG) vesicles to become fully interdigitated. Phospholipid translocation across these interdigitated membranes is significantly inhibited, most likely because of an increase in membrane rigidity. Graphic
ISSN:1472-7862
DOI:10.1016/S1472-7862(01)00005-3