Analysis of Pesticides Residues in Foods by Commercial Kits and a Comparison with Gas Chromatography

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 33; no. 5; pp. 458 - 466_1
• Main Authors: TSUMURA, Yukari, TONOGAI, Yasuhide, NAKAMURA, Yumiko, MIYATA, Masahiro, KAMAKURA, Kazumasa, HASHIHATA, Naoki, IWATA, Kunihiko, ITO, Sumio, MINABA, Kiyomi, OKI, Yoshinori, KODAMA, Mitsuo, ITO, Yoshio
• Format: Journal Article
• Language: English
• Published: Japanese Society for Food Hygiene and Safety 1992
• Subjects: acetylcholinesterase-inhibiting pesticide; aldicarb; carbofuran; commercial kit for pesticide residue analysis; enzyme linked immunosorbent assay (ELISA); gas chromatography; pesticide
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.33.458

Commercial analysis kits for acetylcholinesterase-inhibiting pesticides (AI) and enzyme-linked immunosorbent assay (ELISA) have been examined in comparison with gas chromatographic (GC) analysis. The AI kit could detect N-methylcarbamate pesticides with better sensitivity than GC analysis. However clean-up of the sample solution prior to detection was required. Carbofuran was detected by ELISA in tubes with recovery in the range of 57-159% and a detection limit of 6ppb, which was about 10 times better than GC analysis. On the other hand, ELISA for aldicarb in wells gave a detection limit of 3ppb, which was 20 times better than GC analysis. However, it gave positive and negative errors in the case of wheat and pumpkin, respectively.