Response of petroleum-contaminated soil to chemical oxidation combined with biostimulation

• Published in: Ecotoxicology and environmental safety Vol. 282; p. 116694
• Main Authors: Huang, Yongjie, Zhou, Zhenzhen, Cai, Yuting, Li, Xinmeng, Huang, Yufeng, Hou, Jinyu, Liu, Wuxing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.09.2024; Elsevier
• Subjects: Arthrobacter; Bacteria - drug effects; Bacteria - genetics; Biodegradation, Environmental; Biostimulation; Chemical oxidation; Environmental Restoration and Remediation - methods; Fertilizers - analysis; Hydrocarbons; Microbial community; Oil-contaminated soil; Oxidation-Reduction; Petroleum; Sodium Compounds - toxicity; Soil - chemistry; Soil Microbiology; Soil Pollutants - analysis; Soil Pollutants - toxicity; Sulfates; Oil-contaminated soil; Biostimulation; Chemical oxidation; Microbial community
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116694

In this study, a microcosm experiment was conducted to investigate the effects of Na2S2O8 preoxidation combined with biostimulation on petroleum-contaminated soil remediation. The response of microbial community during this process was explored using BIOLOG ECO microplate carbon utilization method and 16 s rDNA high-throughput sequencing. The results showed that use of 10 mg/g Na2S2O8 removed 19.8 % of the petroleum hydrocarbons, reduced soil biotoxicity and did not affect soil microbial activity compared to other concentrations. Therefore, sodium persulfate of ca. 10 mg/g was used to oxidize petroleum in soil before the biostimulation experiment with organic and inorganic fertilizers. Our finding showed that the content of total petroleum hydrocarbons (TPHs) in soil was reduced by 43.3 % in inorganic fertilizer treatment after 60 days. The results of BIOLOG ECO microplate carbon utilization analysis and 16 S rDNA high-throughput sequencing further confirmed that biostimulation quickly restored the microbial activities in oxidant treated soil. The main marker bacteria in chemical oxidation combined with biostimulation remediation were Arthrobacter and Paenarthrobacter, and their relative abundances were both significantly negatively correlated with the content of petroleum hydrocarbons in soil. [Display omitted] •The contaminated soil was pre-oxidized with sodium persulfate (Na2S2O8).•Changes of soil microbial community structure during peroxidation.•The main markers of soil microorganisms were arthrobacter and Arthrobacter was Arthrobacter and Paenarthrobacter.•Effects of different fertilizer treatments on the removal of petroleum hydrocarbons.