In vitro metabolism of steroids by human and rabbit submaxillary glands

Human and rabbit submaxillary gland homogenates were incubated in vitro for 2 hr with different labeled substrates under an air atmosphere with a NADPH generating system. Both tissues converted progesterone-4- 14C to 20β-dihydroprogesterone to the extent of 9.7–17.3% for human and 9.5–15.7% for rabb...

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Bibliographic Details
Published inJournal of steroid biochemistry Vol. 2; no. 1; pp. 67 - 76
Main Authors Cardinali, Daniel P., Denari, Horacio, Rosner, Jorge M.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 1971
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Summary:Human and rabbit submaxillary gland homogenates were incubated in vitro for 2 hr with different labeled substrates under an air atmosphere with a NADPH generating system. Both tissues converted progesterone-4- 14C to 20β-dihydroprogesterone to the extent of 9.7–17.3% for human and 9.5–15.7% for rabbit tissue. When pregnenolone-4- 14C was incubated with rabbit submaxillary gland, 20β-dihydropreg-nenolone was detected with a yield of 1.7–4.4%. The labeled pregnenolone was recovered unchanged in human tissue experiments. Both human and rabbit submaxillary glands converted androstenedione-4- 14C to testosterone to the extent of 19.1–31.1% and 9.9–16.5%, respectively. No differences between sexes were observed in either human or rabbit experiments and no radioactivity was associated with the phenolic fractions.
ISSN:0022-4731
DOI:10.1016/0022-4731(71)90010-0