Maturation and germination of mango somatic embryos
Maturation and germination of mango (Mangifera indica L.) somatic embryos were achieved by sequential transfer of heart-shaped somatic embryos on a series of media based on the B-5 formulation. Precocious germination and other developmental abnormalities were controlled by incorporating 3 MU abscisi...
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Published in | Journal of the American Society for Horticultural Science Vol. 114; no. 5 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
01.09.1989
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Subjects | |
Online Access | Get more information |
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Summary: | Maturation and germination of mango (Mangifera indica L.) somatic embryos were achieved by sequential transfer of heart-shaped somatic embryos on a series of media based on the B-5 formulation. Precocious germination and other developmental abnormalities were controlled by incorporating 3 MU abscisic acid (ABA) and 6% (w/v) sucrose in the medium. Other factors, including the substitution of Gelrite (0.175%) for agar, the use of solid rather than liquid medium, and the culture of somatic embryos in darkness until physiological maturity also affected normal growth and development. Coconut water (20%, v/v) was also essential for mango somatic embryogeny. Germinated mango somatic embryos were successfully established in planting medium and they have continued to grow in a greenhouse |
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Bibliography: | F60 9017503 |
ISSN: | 0003-1062 2327-9788 |
DOI: | 10.21273/JASHS.114.5.837 |