Maturation and germination of mango somatic embryos

Maturation and germination of mango (Mangifera indica L.) somatic embryos were achieved by sequential transfer of heart-shaped somatic embryos on a series of media based on the B-5 formulation. Precocious germination and other developmental abnormalities were controlled by incorporating 3 MU abscisi...

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Bibliographic Details
Published inJournal of the American Society for Horticultural Science Vol. 114; no. 5
Main Authors DeWald, S.G. (Fruit Crops Department, Gainesville, FL), Litz, R.E, Moore, G.A
Format Journal Article
LanguageEnglish
Published 01.09.1989
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Summary:Maturation and germination of mango (Mangifera indica L.) somatic embryos were achieved by sequential transfer of heart-shaped somatic embryos on a series of media based on the B-5 formulation. Precocious germination and other developmental abnormalities were controlled by incorporating 3 MU abscisic acid (ABA) and 6% (w/v) sucrose in the medium. Other factors, including the substitution of Gelrite (0.175%) for agar, the use of solid rather than liquid medium, and the culture of somatic embryos in darkness until physiological maturity also affected normal growth and development. Coconut water (20%, v/v) was also essential for mango somatic embryogeny. Germinated mango somatic embryos were successfully established in planting medium and they have continued to grow in a greenhouse
Bibliography:F60
9017503
ISSN:0003-1062
2327-9788
DOI:10.21273/JASHS.114.5.837