Energetic costs of mounting an immune response in a coral reef damselfish ( Pomacentrus amboinensis )

• Published in: Canadian journal of zoology Vol. 102; no. 4; pp. 380 - 392
• Main Authors: Levet, Marie, Roche, Dominique G., Killen, Shaun S., Colosio, Simona, Bshary, Redouan, Miest, Joanna J., Binning, Sandra A.
• Format: Journal Article
• Language: English
• Published: Ottawa Canadian Science Publishing NRC Research Press 01.04.2024
• Subjects: Coral reefs; Endotoxins; Exposure; Gene expression; Immune response; Immune system; Injection; Lipopolysaccharides; Metabolic rate; Metabolism; Oxygen consumption; Oxygen uptake; Pomacentrus amboinensis; Respirometry
• ISSN: 0008-4301; 1480-3283; 1480-3283; 0008-4301
• DOI: 10.1139/cjz-2023-0033

While immune responses can be energetically costly, quantifying these costs is challenging. We tested the metabolic costs of immune activation in damselfish ( Pomacentrus amboinensis Bleeker, 1868) following a mass-adjusted injection of lipopolysaccharide (LPS) endotoxin. Fish were divided into eight treatments: two controls (handling and saline injection) and six LPS groups with concentrations ranging from 3 to 100 mg kg −1 . We used intermittent flow respirometry to measure differences in oxygen uptake (delta ṀO 2 ) 20 h before versus 20 h after LPS injection and changes in metabolic traits (lowest, routine, and peak metabolic rates) as proxies of the aerobic costs of metabolism. Spleen somatic index (SSI) and gene expression in spleens were measured to assess immune activation. We found no difference in metabolic traits or SSI but observed different nonlinear patterns of delta ṀO 2 in fish exposed to 50 and 100 mg kg −1 LPS compared to lower doses and controls. Fish exposed to high doses of LPS also had lower residual aerobic scope compared to controls and lower LPS doses. Fish exposed to doses of 3, 50, and 100 mg kg −1 showed altered gene expression compared to the handling control. Overall, our results suggest that immune activation has measurable effects on metabolic traits that are both dose- and time-dependent.