Effect of hemic hypoxia on dynamics of GFAP concentrations in the structures of the brain and blood serum of rats

• Published in: Visnyk Dnipropetrovsʹkoho universytetu. Serii͡a︡ biolohii͡a︡, ekolohiia Vol. 24; no. 1; pp. 143 - 150
• Main Authors: T. I. Duka, V. I. Chоrna
• Format: Journal Article
• Language: English
• Published: Oles Honchar Dnipropetrovsk National University 02.03.2016
• Subjects: astroglial intermediate filaments; blood; ELISA
• ISSN: 2310-0842; 2312-301X
• DOI: 10.15421/011617

This article clarifies the questions on study of hypoxic influence on distribution of filament and soluble forms of GFAP in various structures of the brain (neocortex, cerebellum, hippocampus, striatum, middle brain, pons) and blood of the rats. Quantitative analysis of the contents of GFAP in the brain structures of hypoxic rats has established that hemic hypoxia results in changes in intracellular levels of GFAP forms and also in updating their ratio, which allows one to assume not only a change in astroglial cells, but also testifies to reorganization in the system of intermediate filaments of astrocytes. The level of GFAP substantially changed in all cerebral formations, which was already investigated in the early terms of hypoxic period. Observations showed that hemic hypoxia exerted a varied influence on expression of neurospecific protein in the different structures of cerebrum of rats. Differences in expression of GFAP can be caused by the regional differences in astroglial cellular population, and also their internal features that define the possible answers to hypoxic damage in different functional and morphological structures of the brain. An increase in expression of the investigated form of protein can explain strengthening of astroglial reactivity, a feature of the brain that appears in various types of pathologies of the CNS. Reactive asters in such exhibit hypertrophy and are characterized by an increased level of GFAP, which is an early and reliable indicator of astroglial pathology. An increase in expression of the investigated form of protein may be explained by strengthening of astroglial reactivity, a feature of the brain that appears in various types of pathologies of the CNS. The contents of GFAP in the blood of adult rats, as a result of the hypoxic influence received from it, can indicate a release of GFAP from damaged astrocytes in the blood flow.