Comparative starch-gel electrophoresis of liver esterases from seven species

1.1. Different electrophoretic patterns of bands with enzymatic activities were obtained for esterases of beef, pork, sheep, Rh monkey, chicken, turkey and human livers. Patterns for human and monkey liver esterases showed striking similarities.2.2. At a constant voltage and starch concentration, a...

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Published inComparative biochemistry and physiology. B, Comparative biochemistry Vol. 39; no. 3; pp. 483,IN1,485 - 484,IN4,488
Main Authors Mendoza, C.E., Hatina, G.V.
Format Journal Article
LanguageEnglish
Published England Elsevier Inc 15.07.1971
Subjects
Acetates
Animals
Bromine
Cattle
Chickens
Electrophoresis
Esterases - isolation & purification
Female
Fluorescence
Gels
Haplorhini
Humans
Hydrochloric Acid
Hydrogen-Ion Concentration
Indoles
Liver - enzymology
Macaca
Male
Maleates
Methods
Sheep
Species Specificity
Staining and Labeling
Starch
Swine
Tromethamine
Turkeys
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Summary:1.1. Different electrophoretic patterns of bands with enzymatic activities were obtained for esterases of beef, pork, sheep, Rh monkey, chicken, turkey and human livers. Patterns for human and monkey liver esterases showed striking similarities.2.2. At a constant voltage and starch concentration, a slight change in pH and/or buffer concentration brought about a marked change in electrophoregrams.3.3. Adequate resolution of a beef and chicken esterase mixture and differentiation of human and Rh monkey liver esterases were achieved.4.4. Patterns of the fluorescent bands detectable with 5-bromoindoxyl acetate were consistently obtained for each species. No further attempt was made to characterize the bands.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-0491
DOI:10.1016/0305-0491(71)90193-3