Immunohistochemical Study of Acute Effects of Ethanol on Midbrain of Wistar Rats Associated with the Date of Birth of Neurons in Encephalon

• Published in: International journal of morphology Vol. 27; no. 3; pp. 677 - 682
• Main Authors: Lopes, Danielly Bandeira, Nascimento, Guilherme Nobre Lima do, Carvalho, Adryano Augustto Valladão de, Neto, Advaldo Carlos de Souza, Lima, Natácia Evangelista de, Aversi-Ferreira, Tales Alexandre
• Format: Journal Article
• Language: English
• Published: Sociedad Chilena de Anatomía 01.09.2009
• Subjects: ANATOMY & MORPHOLOGY; Mesencéfalo; Brain; Neuronal migration; Fetal Alcohol Syndrome; Síndrome de Alcoholismo Fetal; Ethanol; Etanol; Midbrain; Cerebro; Migración neuronal
• ISSN: 0717-9502; 0717-9502
• DOI: 10.4067/S0717-95022009000300009

The verification of the acute effects of ethanol on the midbrain, which neurons are mainly formed in E14, will ratify if the acute effects of ethanol are associated, in terms of prevalence, to the date of birth of neurons in certain regions of the brain. The aim of this study was to determine the effects of acute exposure to ethanol on the neuronal density of the midbrain and to associate these results with acute effects of ethanol in other regions of the brain under the same conditions. The rats were treated with intraperitoneal ethanol during one day (E12) and 1h after the last ethanol injection was applied to BrdU. The animals were sacrificed; the brains were removed and sectioned. The sections were treated with DAB for the BrdU revelation, the slides were stained with safranin for one group and another group was stained with H & E. The effects of acute injection of ethanol in E12 on the midbrain were not detected in this study. Data from literature suggest that the deleterious action of acute ethanol consumption only occur if the drug is injected at times near the beginning of the migration of neurons in that particular region of the brain.