Genomic landscape of human erythroleukemia K562 cell line, as determined by next-generation sequencing and cytogenetics

• Published in: Acta haematologica polonica Vol. 48; no. 4; pp. 343 - 349
• Main Authors: Kurkowiak, Małgorzata, Pępek, Monika, Machnicki, Marcin M., Solarska, Iwona, Borg, Katarzyna, Rydzanicz, Małgorzata, Stawiński, Piotr, Płoski, Rafał, Stokłosa, Tomasz
• Format: Journal Article
• Language: English
• Published: Elsevier Urban & Partner Sp. z o.o 01.10.2017
• Subjects: Chronic myeloid leukemia; Genomic instability; Human erythroleukemia; K562; Next-generation sequencing; Human erythroleukemia; Next-generation sequencing; Chronic myeloid leukemia; K562; Genomic instability

We have performed detailed analysis of the genomic landscape of commercially available K562 cells, employing targeted enrichment of nearly 1300 cancer-related genes followed by next-generation sequencing (NGS) and also classical cytogenetics. Deep sequencing revealed 88 variants of potentially biolo...