Genomic landscape of human erythroleukemia K562 cell line, as determined by next-generation sequencing and cytogenetics

• Published in: Acta haematologica polonica Vol. 48; no. 4; pp. 343 - 349
• Main Authors: Kurkowiak, Małgorzata, Pępek, Monika, Machnicki, Marcin M., Solarska, Iwona, Borg, Katarzyna, Rydzanicz, Małgorzata, Stawiński, Piotr, Płoski, Rafał, Stokłosa, Tomasz
• Format: Journal Article
• Language: English
• Published: Elsevier Urban & Partner Sp. z o.o 01.10.2017
• Subjects: Chronic myeloid leukemia; Genomic instability; Human erythroleukemia; K562; Next-generation sequencing; Human erythroleukemia; Next-generation sequencing; Chronic myeloid leukemia; K562; Genomic instability
• ISSN: 0001-5814
• DOI: 10.1016/j.achaem.2017.06.002

We have performed detailed analysis of the genomic landscape of commercially available K562 cells, employing targeted enrichment of nearly 1300 cancer-related genes followed by next-generation sequencing (NGS) and also classical cytogenetics. Deep sequencing revealed 88 variants of potentially biological significance. Among them we have detected alterations in genes already known to be mutated in K562, such as TP53 but also in several other genes, which are implicated in tumorigenesis and drug resistance, such as MLH1, ASXL1 and BRCA1 as the most prominent examples. Fluorescence in situ hybridization (FISH) of interphases of K562 cells revealed multiplication of the BCR and ABL1 gene copies, as well as the amplification of the BCR-ABL1 fusion gene. Our results may help to better understand genomic instability of the blastic phase of CML represented by the K562 cell line and can help researchers who want to employ this cell line in various experimental settings.