Genomic landscape of human erythroleukemia K562 cell line, as determined by next-generation sequencing and cytogenetics

We have performed detailed analysis of the genomic landscape of commercially available K562 cells, employing targeted enrichment of nearly 1300 cancer-related genes followed by next-generation sequencing (NGS) and also classical cytogenetics. Deep sequencing revealed 88 variants of potentially biolo...

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Published inActa haematologica polonica Vol. 48; no. 4; pp. 343 - 349
Main Authors Kurkowiak, Małgorzata, Pępek, Monika, Machnicki, Marcin M., Solarska, Iwona, Borg, Katarzyna, Rydzanicz, Małgorzata, Stawiński, Piotr, Płoski, Rafał, Stokłosa, Tomasz
Format Journal Article
LanguageEnglish
Published Elsevier Urban & Partner Sp. z o.o 01.10.2017
Subjects
Chronic myeloid leukemia
Genomic instability
Human erythroleukemia
K562
Next-generation sequencing
Human erythroleukemia
Next-generation sequencing
Chronic myeloid leukemia
K562
Genomic instability
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Abstract We have performed detailed analysis of the genomic landscape of commercially available K562 cells, employing targeted enrichment of nearly 1300 cancer-related genes followed by next-generation sequencing (NGS) and also classical cytogenetics. Deep sequencing revealed 88 variants of potentially biological significance. Among them we have detected alterations in genes already known to be mutated in K562, such as TP53 but also in several other genes, which are implicated in tumorigenesis and drug resistance, such as MLH1, ASXL1 and BRCA1 as the most prominent examples. Fluorescence in situ hybridization (FISH) of interphases of K562 cells revealed multiplication of the BCR and ABL1 gene copies, as well as the amplification of the BCR-ABL1 fusion gene. Our results may help to better understand genomic instability of the blastic phase of CML represented by the K562 cell line and can help researchers who want to employ this cell line in various experimental settings.
AbstractList We have performed detailed analysis of the genomic landscape of commercially available K562 cells, employing targeted enrichment of nearly 1300 cancer-related genes followed by next-generation sequencing (NGS) and also classical cytogenetics. Deep sequencing revealed 88 variants of potentially biological significance. Among them we have detected alterations in genes already known to be mutated in K562, such as TP53 but also in several other genes, which are implicated in tumorigenesis and drug resistance, such as MLH1, ASXL1 and BRCA1 as the most prominent examples. Fluorescence in situ hybridization (FISH) of interphases of K562 cells revealed multiplication of the BCR and ABL1 gene copies, as well as the amplification of the BCR-ABL1 fusion gene. Our results may help to better understand genomic instability of the blastic phase of CML represented by the K562 cell line and can help researchers who want to employ this cell line in various experimental settings.
Author Stawiński, Piotr
Rydzanicz, Małgorzata
Kurkowiak, Małgorzata
Pępek, Monika
Solarska, Iwona
Machnicki, Marcin M.
Borg, Katarzyna
Stokłosa, Tomasz
Płoski, Rafał
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  email: tomasz.stoklosa@wum.edu.pl
  organization: Department of Immunology, Center of Biostructure Research, Medical University of Warsaw, Poland
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CitedBy_id crossref_primary_10_1016_j_tiv_2023_105608
crossref_primary_10_3390_ijms24065377
crossref_primary_10_3390_genes11101145
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Keywords Human erythroleukemia
Next-generation sequencing
Chronic myeloid leukemia
K562
Genomic instability
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Snippet We have performed detailed analysis of the genomic landscape of commercially available K562 cells, employing targeted enrichment of nearly 1300 cancer-related...
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SubjectTerms Chronic myeloid leukemia
Genomic instability
Human erythroleukemia
K562
Next-generation sequencing
Title Genomic landscape of human erythroleukemia K562 cell line, as determined by next-generation sequencing and cytogenetics
URI https://dx.doi.org/10.1016/j.achaem.2017.06.002
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