Bimetallic Fe-Co Nanoalloy Confined in Porous Carbon Skeleton with Enhanced Peroxidase Mimetic Activity for Multiple Biomarkers Monitoring

• Published in: Journal of analysis and testing Vol. 7; no. 1; pp. 53 - 68
• Main Authors: Luo, Jingjing, Liu, Ruilin, Zhao, Shuchang, Gao, Yuan
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.03.2023
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Monitoring/Environmental Analysis; Original Paper; Carbon nanozyme; Colorimetric assay; Fe-Co nanoalloy; Glucose; Glutathione
• ISSN: 2096-241X; 2509-4696
• DOI: 10.1007/s41664-022-00241-z

Carbon-based nanozymes with multifunctional applications have attracted enormous attention, however, there is still a lack of an effective strategy for inexpensive preparation of high-active carbon-based nanozyme. We herein report a waste paper-derived CoFe 2 O 4 /porous carbon nanozyme for the colorimetric detection of glucose and glutathione (GSH) in biofluids. The hybrid CoFe 2 O 4 /porous carbon material was successfully prepared via a combined impregnation, hydrolysis and carbonization method. Newly constructed CoFe 2 O 4 /porous carbon material with enhanced peroxidase-like activity could oxidize colorless tetramethylbenzidine (TMB) to blue oxidized TMB with adding H 2 O 2 . A sensitive colorimetric analysis platform for monitoring the levels of glucose and GSH in biofluids was respectively developed. The proposed analytical method possessed predominant features such as low limit of detections (LODs) (0.16 µmol/L for glucose and 120 nmol/L for GSH), broad linear ranges (1–200 µmol/L for glucose and 1–50 µmol/L for GSH), and excellent practical potential. This multipurpose platform not only provides a promising strategy for transforming waste paper into valuable carbon-based nanozyme, but also paves the way for excavating the potential biomedical and environmental applications from waste paper.