Factores que afectan la tasa de gestación con embriones cebú producidos in vitro en trópico

Objective. To study the factors affecting the success of embryo transfers (ET). Materials and methods. 1087 ET in zebu cattle were analyzed. The considered extrinsic factors of the donor cows were the cattle production unit from which they were sourced (n=13), amount of transfers in the unit  (1 tim...

Full description

Saved in:
Bibliographic Details
Published inRevista MVZ (Medicina Veterinaria y Zootecnia) Vol. 29; no. 2; p. e3453
Main Authors Alpirez Mendoza, Manlio, Rodríguez Andrade, Araceli, Román Fernández, Orlando, Barrientos Morales, Manuel, Zavaleta Martínez, Alondra, Hernández Beltrán, Antonio, Avendaño Reyes, Leonel, Cervantes Acosta, Patricia, Domínguez Mancera, Belisario
Format Journal Article
LanguageSpanish
English
Published Universidad de Cordoba 01.05.2024
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:Objective. To study the factors affecting the success of embryo transfers (ET). Materials and methods. 1087 ET in zebu cattle were analyzed. The considered extrinsic factors of the donor cows were the cattle production unit from which they were sourced (n=13), amount of transfers in the unit  (1 time, >1 time), geographical zone (n=4), period (dry or rainy) and year season; and the intrinsic factors were the breed of the bull and DC, semen, embryo quality  and embryo developmental stage. Statistics were compiled with ci2 for the analysis of the main effects, and correlations were estimated through a multivariate clustering analysis. Results. The general pregnancy rate was 37.99±1.47, and there were differences (p<0.05) observed among the CPU (2.78–75%). The PR was higher in the dry than in the rainy period (44.55 vs 31.28%), and in fall and winter (p<0.05) than spring and summer (42.76, 43.98, 37.57 and 29.77%, respectively), and there were differences observed between the geographical zones as well (p<0.05). The PR was higher with excellent-quality embryos (p<0.05) than with good-quality embryos (38.9 vs. 26.3%). The period and year season had an effect over the RC (p<0.05), and the grouping analysis showed a correlation (r>0.90) between the period and year season during Oocyte pick-up (OPU) over the PR; moreover, semen, EQ and EDS had larger associations with the PR (r>0.80). Conclusions. Environmental conditions at the moment of OPU and EQ are factors worth considering in embryo transfer programs with zebu cattle using in vitro produced embryos in tropical conditions. Objetivo. Estudiar los factores que afectan el éxito de la transferencia de embriones. Materiales y métodos. Se analizaron 1087 TE de raza cebú. Los factores extrínsecos de la donadora bovina (DB) fueron: unidad de producción pecuaria (UPP) (n=13), veces que realizan TE en UPP (1 vez, >1 vez), región/zona (n=4), época (seca o lluvia) y estación del año; e intrínsecos: raza de donadora y toro, semen (convencional o sexado), calidad y desarrollo embrionario (DE). En la receptora bovina (RB): tamaño del cuerpo lúteo (CL1, CL2 y CL3), época (seca o lluvia) y estación. La estadística se realizó con ci2 para el análisis de los efectos principales y multivariado de agrupamiento para estimar las asociaciones. Resultados. La tasa de gestación (TG) general fue 37.99±1.47, hubo diferencias (p<0.05) entre las UPP (2.78–75%), la época seca fue mayor que lluvia (44.55 vs 31.28%), otoño e invierno (p<0.05) en comparación con primavera y verano (42.76, 43.98, 37.57 y 29.77%, respectivamente) y la región/zona fueron diferentes (p<0.05). La CE excelente presentó mayor (p<0.05) TG que la buena (38.9 vs. 26.3%). En la RB, la época y la estación del año tuvieron efecto (p<0.05). El análisis de agrupamiento mostro asociación (r>0.90) de estación con época durante la aspiración folicular transvaginal guiada por ultrasonografía OPU con la TG; el semen, CE y DE tuvieron una mayor asociación con TG (r>0.80). Conclusiones. Los factores ambientales al momento de la OPU y la CE son efectos por considerar en programas de TE cebú producidos in vitro en el trópico. 
ISSN:0122-0268
1909-0544
1909-0544
DOI:10.21897/rmvz.3453