Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai

The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed...

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Bibliographic Details
Published inNatural product sciences Vol. 24; no. 4; pp. 241 - 246
Main Authors Nugroho, Agung, Park, Kyoung-Sik, Seo, Dong-Jin, Park, Hee-Juhn
Format Journal Article
LanguageEnglish
Published 한국생약학회 2018
Subjects
약학
Lamiaceae
rutin
ecdysone
HPLC
Lamium takesimense Nakai
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Summary:The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 mm, 4.6 mm ? 250 mm) with a gradient elution of H2O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order: lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g). KCI Citation Count: 0
ISSN:1226-3907
2288-9027
DOI:10.20307/NPS.2018.24.4.241