Welly: a web-tool for visualizing growth curves from microplate data

Welly is a web-based tool designed to simplify the visualization and analysis of growth curves from 96- and 384-well plates, addressing the limitations of existing commercial and coding-based solutions. Users can upload plate reader data in CSV or Excel format, easily select sample names and replica...

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Published inBioinformatics advances Vol. 5; no. 1; p. vbaf038
Main Authors Meier, Felix, Williams, Tom, Paulsen, Ian
Format Journal Article
LanguageEnglish
Published England 2025
Online AccessGet full text
ISSN2635-0041
2635-0041
DOI10.1093/bioadv/vbaf038

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Abstract Welly is a web-based tool designed to simplify the visualization and analysis of growth curves from 96- and 384-well plates, addressing the limitations of existing commercial and coding-based solutions. Users can upload plate reader data in CSV or Excel format, easily select sample names and replicates and Welly generates interactive growth curves displaying the mean and standard deviation of triplicates. Additional features include heat map visualizations of maximum values, and downloadable interactive graphs of publication-quality figures and statistics files containing area under curve and max growth rate value of replicates. Welly is freely available at https://synbioexplorer.pythonanywhere.com, providing an easy-to-use interface accessible to all. All the code is publicly available at the github repository https://github.com/SynBioExplorer/Welly under the MIT license. The website will remain freely accessible for at least 2 years post publication, likely longer.
AbstractList Welly is a web-based tool designed to simplify the visualization and analysis of growth curves from 96- and 384-well plates, addressing the limitations of existing commercial and coding-based solutions. Users can upload plate reader data in CSV or Excel format, easily select sample names and replicates and Welly generates interactive growth curves displaying the mean and standard deviation of triplicates. Additional features include heat map visualizations of maximum values, and downloadable interactive graphs of publication-quality figures and statistics files containing area under curve and max growth rate value of replicates.SummaryWelly is a web-based tool designed to simplify the visualization and analysis of growth curves from 96- and 384-well plates, addressing the limitations of existing commercial and coding-based solutions. Users can upload plate reader data in CSV or Excel format, easily select sample names and replicates and Welly generates interactive growth curves displaying the mean and standard deviation of triplicates. Additional features include heat map visualizations of maximum values, and downloadable interactive graphs of publication-quality figures and statistics files containing area under curve and max growth rate value of replicates.Welly is freely available at https://synbioexplorer.pythonanywhere.com, providing an easy-to-use interface accessible to all. All the code is publicly available at the github repository https://github.com/SynBioExplorer/Welly under the MIT license. The website will remain freely accessible for at least 2 years post publication, likely longer.Availability and implementationWelly is freely available at https://synbioexplorer.pythonanywhere.com, providing an easy-to-use interface accessible to all. All the code is publicly available at the github repository https://github.com/SynBioExplorer/Welly under the MIT license. The website will remain freely accessible for at least 2 years post publication, likely longer.
Welly is a web-based tool designed to simplify the visualization and analysis of growth curves from 96- and 384-well plates, addressing the limitations of existing commercial and coding-based solutions. Users can upload plate reader data in CSV or Excel format, easily select sample names and replicates and Welly generates interactive growth curves displaying the mean and standard deviation of triplicates. Additional features include heat map visualizations of maximum values, and downloadable interactive graphs of publication-quality figures and statistics files containing area under curve and max growth rate value of replicates. Welly is freely available at https://synbioexplorer.pythonanywhere.com, providing an easy-to-use interface accessible to all. All the code is publicly available at the github repository https://github.com/SynBioExplorer/Welly under the MIT license. The website will remain freely accessible for at least 2 years post publication, likely longer.
Author Williams, Tom
Paulsen, Ian
Meier, Felix
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Cites_doi 10.1128/msystems.00508-21
10.1186/s13104-021-05573-0
10.1186/s12859-024-05692-y
10.1186/s12859-024-05817-3
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