Welly: a web-tool for visualizing growth curves from microplate data

• Published in: Bioinformatics advances Vol. 5; no. 1; p. vbaf038
• Main Authors: Meier, Felix, Williams, Tom, Paulsen, Ian
• Format: Journal Article
• Language: English
• Published: England 2025
• ISSN: 2635-0041; 2635-0041
• DOI: 10.1093/bioadv/vbaf038

Welly is a web-based tool designed to simplify the visualization and analysis of growth curves from 96- and 384-well plates, addressing the limitations of existing commercial and coding-based solutions. Users can upload plate reader data in CSV or Excel format, easily select sample names and replicates and Welly generates interactive growth curves displaying the mean and standard deviation of triplicates. Additional features include heat map visualizations of maximum values, and downloadable interactive graphs of publication-quality figures and statistics files containing area under curve and max growth rate value of replicates. Welly is freely available at https://synbioexplorer.pythonanywhere.com, providing an easy-to-use interface accessible to all. All the code is publicly available at the github repository https://github.com/SynBioExplorer/Welly under the MIT license. The website will remain freely accessible for at least 2 years post publication, likely longer.