Measurement of polyclonal and antigen-specific cytotoxic T cell function

Measurement of in vitro cytotoxic function of human T cells can be accomplished by polyclonal stimulation of T cell effectors using anti-CD3 antibody, which stimulates all cytolytic effector cells, or with a specific stimulating antigen. Accordingly, two sets of assays of cytolytic T cell function a...

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Bibliographic Details
Published inCurrent protocols in immunology Vol. Chapter 7; p. Unit 7.17
Main Authors Biddison, W E, Lichtenfels, R, Adibzadeh, M, Martin, R
Format Journal Article
LanguageEnglish
Published United States 01.05.2001
Subjects
CD3 Complex - immunology
Cytotoxicity Tests, Immunologic - methods
Epitopes, T-Lymphocyte - analysis
Humans
T-Lymphocytes, Cytotoxic - immunology
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Summary:Measurement of in vitro cytotoxic function of human T cells can be accomplished by polyclonal stimulation of T cell effectors using anti-CD3 antibody, which stimulates all cytolytic effector cells, or with a specific stimulating antigen. Accordingly, two sets of assays of cytolytic T cell function are described in this unit, one for measuring anti-CD3-mediated cytotoxicity and the other for measuring antigen-specific cytotoxicity. Although the calcein release assay (CARE-LASS) described here is for use with antigen-activated cytotoxic T lymphocytes (CTL) as well as natural killer (NK) or lymphokine-activated killer (LAK) cells, minor changes in the protocols that address polyclonal T cell activation are described that make them suitable for use with calcein-labeled target cells.
ISSN:1934-368X
DOI:10.1002/0471142735.im0717s17