Ca2+-nano starch-lutein endowed 3D printed surimi with antioxidation and mutual reinforcing transmembrane transport mechanisms via hepg2 and caco-2 cells model

• Published in: Food research international Vol. 191; p. 114691
• Main Authors: Li, Gaoshang, Shi, Rong, Zhan, Junqi, Wu, Yiduo, Wan, Yue, Yao, Qian, Hu, Yaqin, Wu, Chunhua, Yang, Wenge, Wan, Wubo
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.09.2024
• Subjects: Absorption promoting mechanism; Antioxidant properties; Functional inks; Printability; Transmembrane transport; Printability; Antioxidant properties; Absorption promoting mechanism; Transmembrane transport; Functional inks
• ISSN: 0963-9969; 1873-7145; 1873-7145
• DOI: 10.1016/j.foodres.2024.114691

[Display omitted] •3D printed Ca2+-nano starch-lutein-surimi was prepared.•Chemical anti-oxidation and cell anti-oxidation of printed surimi were determined.•Transmembrane absorption and transport of printed surimi were determined.•Mechanism of improved bioavailability of printed functional surimi were manifested. To better enhance printing effects meanwhile casting functionality, antioxidation and absorption of bioactive component in printed Ca2+-nano starch (NS)-lutein (L)-surimi were investigated. Results shown that Ca2+-NS-L promoted surimi printability due to enhanced gel strength and denser structure. Mixing Ca2+-NS-L endowed printed surimi with antioxidation (DPPH, ABTS, hydroxyl radical, Fe2+ reduction were 42 %, 79 %, 65 %, 0.104 mg·mL−1, respectively) due to the ability of lutein with more –OH groups and conjugate bonds to capture free radicals. It also manifested in cellular antioxidation that Ca2+-NS-L-surimi regulated the level of Nrf2 to protect gene expression of antioxidases (SOD, CAT, GSH-Px increased by 30–180 %, compared to damaged cells) through keap1-Nrf2-ARE pathway. Additionally, lutein absorption and transportation of Ca2+-NS-L-surimi increased by 20 %, compared to NS-L. Possibly, combination of samples and membrane was facilitated by surface hydrophobic, promoting endocytosis. Meanwhile, digestive surimi (peptides) with acidic-alkaline amino acids and negative charges made samples be attracted and moved in bypass parts under electrostatic traction and repulsion (electrostatic domain) to promote transport process. Also, Ca2+ facilitated CaM expression in membrane and formed Ca2+ channel by combining with CaM to accelerate entry of samples into cells. Conclusively, Ca2+-NS-L both strengthened printability of surimi and antioxidation, promoting application of printed functional surimi.