Quaternized oxidized sodium alginate injectable hydrogel with high antimicrobial and hemostatic efficacy promotes diabetic wound healing

• Published in: International journal of pharmaceutics Vol. 661; p. 124421
• Main Authors: Ma, Shuang, Chen, Kecheng, Ding, Qiteng, Zhang, Shuai, lu, Yang, Yu, Taojing, Ding, Chuanbo, Liu, Wencong, liu, Shuang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.08.2024
• Subjects: Hemostatic and antibacterial; Quaternized oxidized sodium alginate; Self-healing hydrogel; Self-healing hydrogel; Hemostatic and antibacterial; Quaternized oxidized sodium alginate
• ISSN: 0378-5173; 1873-3476; 1873-3476
• DOI: 10.1016/j.ijpharm.2024.124421

[Display omitted] •Preparation of hydrogels with self-healing injectable properties (QOSA&CMCS&DABP).•Expanded application of antler blood with hydrogel-controlled release of antler blood peptides at the wound site.•QOSA & CMCS & DABP showed better hemostatic properties.•QOSA & CMCS & DABP promote collagen deposition, cell proliferation and tissue regeneration at the wound site.•QOSA & CMCS & DABP regulate the expression of wound healing-related factors. In this paper, a hydrogel material with efficient antibacterial, hemostatic, self-healing, and injectable properties was designed for the treatment of diabetic wounds. Firstly, quaternary ammonium salts were grafted with oxidized sodium alginate, and quaternized oxidized sodium alginate (QOSA) was synthesized. Due to the introduction of quaternary ammonium group it has antibacterial and hemostatic effects, at the same time, due to the presence of aldehyde group it can be reacted with carboxymethyl chitosan (CMCS) to form a hydrogel through the Schiff base reaction. Furthermore, deer antler blood polypeptide (DABP) was loaded into the hydrogel (QOSA&CMCS&DABP), showing good swelling ratio and bacteriostatic effect. In vitro and in vivo experiments demonstrated that the hydrogel not only quickly inhibited hepatic hemorrhage in mice and reduced coagulation index and clotting time in vitro, but also significantly enhanced collagen deposition at the wound site, accelerating wound healing. This demonstrates that the multifunctional hydrogel materials (QOSA&CMCS&DABP) have promising applications in the acceleration of skin wound healing and antibacterial promotion.