Functional characteristics of TG and TnonG cells in a three party MLR

• Published in: The Journal of immunology (1950) Vol. 126; no. 1; pp. 118 - 121
• Main Authors: Gebel, HM, Radaj, PA, Holmes, D, Schwartz, BD, Rodey, GE
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.01.1981
• Subjects: Concanavalin A - pharmacology; Humans; Immunosuppression; Lymphocyte Activation; Lymphocyte Culture Test, Mixed; Receptors, Fc; T-Lymphocytes - classification
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.126.1.118

Human peripheral blood T lymphocytes were separated into highly purified Fc gamma receptor-positive (TG) and negative (TnonG) populations. These subgroups were compared with unseparated T cells for their ability to proliferate in response to concanavalin A or allogeneic cells, and to suppress a mixed leukocyte culture reaction (MLR). TG cells fail to vigorously proliferate in response to Con A or irradiated allogeneic cells. Moreover, the response of TnonG populations to Con A and allogeneic cells is enhanced compared with unseparated T cells, suggesting active suppression by TG cells. The suppressive effects of TG cells was confirmed in a 3-party MLR. In contrast, TnonG cells failed to suppress the MLR. However, Con A-activated TnonG cells did suppress the MLR and were as effective as TG cells.