Mobile ELISA with a Transparent Incubator

Enzyme-linked immunosorbent assay (ELISA) is one of the most important technologies for biochemical testing critical for diagnosis and monitoring of many diseases. Traditional systems for ELISA incubation and reading are expensive and bulky, thus cannot be used at point-of-care or in the field. Here...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2393; p. 329
Main Authors Zhdanov, Arsenii, Chen, Cassidy, Schmitt, Crystal, Tripp, Tyler, Miller, Chase, Pyayt, Anna
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Cell Phone
Enzyme-Linked Immunosorbent Assay
Incubators
Point-of-Care Systems
Telemedicine
mHealth
Biomedical testing
Cortisol
ELISA
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Summary:Enzyme-linked immunosorbent assay (ELISA) is one of the most important technologies for biochemical testing critical for diagnosis and monitoring of many diseases. Traditional systems for ELISA incubation and reading are expensive and bulky, thus cannot be used at point-of-care or in the field. Here, we designed and demonstrated a new miniature mobile phone-based system for ELISA. This mHealth system can be used to complete all steps of the assay, including incubation and reading. It can be fabricated at low cost, it is portable, and can transfer test results via mobile phone. We have designed incubation chamber, imaging enclosure, and data processing algorithm. We demonstrate how mobile ELISA can be calibrated for accurate measurements of cortisol and show successful measurements with the calibrated system. We show that the results acquired with our prototype match well the results obtained with the gold standard.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1803-5_18