ELISA-Based Biosensors

Enzyme-linked immunosorbent assay (ELISA) is by definition a biosensor. However, not all immuno-biosensors involve the use of enzymes, while other biosensors incorporate ELISA as a key signaling component. In this chapter, we review the role of ELISA in signal amplification, integration with microfl...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2612; p. 225
Main Author Matson, Robert S
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Biosensing Techniques
Electrochemical Techniques
Enzyme-Linked Immunosorbent Assay
Lab-On-A-Chip Devices
Luminescent Measurements
Microfluidics
Bio-recognition
Digital ELISA (dELISA)
Digital droplet ELISA (ddELISA)
Ruthenium
Quantum dots
Enzyme-labeled fluorescence (ELF)
Liposome
Digital
Lab-on-a-chip (LOC)
Limit of detection (LOD)
Tyramide (TSA)
Transducer
Amperometric
Potentiometric
Minimal detectable dose (MDD)
Analog
Biosensor
Electrochemiluminescence (ECL)
Luminol
Impedance
Pre-equilibrium digital ELISA (PEdELISA)
Working electrode
Microfluidics
Electrochemical
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Summary:Enzyme-linked immunosorbent assay (ELISA) is by definition a biosensor. However, not all immuno-biosensors involve the use of enzymes, while other biosensors incorporate ELISA as a key signaling component. In this chapter, we review the role of ELISA in signal amplification, integration with microfluidic systems, digital labeling, and electrochemical detection.
ISSN:1940-6029
DOI:10.1007/978-1-0716-2903-1_16