The Oncogenic Potential of Thermotolerant C3H10T 1/2 Cells

• Published in: Nihon Haipāsāmia Gakkai shi Vol. 4; no. 4; pp. 265 - 271
• Main Authors: Komatsu, Kenshi, Okumura, Yutaka, Kodama, Seiji
• Format: Journal Article
• Language: English
• Published: Japanese Society for Thermal Medicine 1988
• Subjects: C3H10T 1/2 cells; Cell transformation; Thermotolerance
• ISSN: 0911-2529; 1881-9516
• DOI: 10.3191/thermalmedicine.4.265

The effects of thermotolerance on cis-platinum induced-oncogenesis were studied using C3H10T 1/2 cell transformation assay system. Exponentially growing cells were exposed to heat at 44°C or 2 μg/ml of cis-platinum with interval time of incubation at 37°C after a priming heat treatment at 44°C for 1 hr. The thermotolerance was developed and continued over several days. Heat sensitized the cytotoxicity to cis-platinum, depending on interval times between both treatments. At 48 hrs after the priming heat exposure, cells losed the sensitization of cell killing to cis-platinum, but were still thermotolerant. Our results indicated that transformation incidence induced by cis-platinum, of these thermotolerant cells, was the same level as that of non-thermotolerant cells. It is suggested that the oncogenesity by cis-platinum in clinical hyperthermia will not be enhanced during the thermotolerant state of cells.