Transfection of Small Noncoding RNAs into Arabidopsis thaliana Protoplasts

Polyethylene glycol transfection of plant protoplasts represents an efficient method to incorporate foreign DNA and study transient gene expression. Here, we describe an optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts. An example of application is provided by...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2166; p. 413
Main Authors Lalande, Stéphanie, Chery, Marjorie, Ubrig, Elodie, Hummel, Guillaume, Kubina, Julie, Geldreich, Angèle, Drouard, Laurence
Format Journal Article
LanguageEnglish
Published United States 2020
Subjects
Alanine - genetics
Arabidopsis - genetics
Protoplasts - metabolism
RNA, Plant - genetics
RNA, Small Untranslated - genetics
RNA, Transfer - genetics
Transfection - methods
Northern blot
Transfection
Small noncoding RNAs
tRNA-derived fragments
Protoplasts
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Summary:Polyethylene glycol transfection of plant protoplasts represents an efficient method to incorporate foreign DNA and study transient gene expression. Here, we describe an optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts. An example of application is provided by demonstrating the incorporation of a 20 nt long small noncoding RNA deriving from the 5' extremity of an A. thaliana cytosolic alanine tRNA into freshly isolated protoplasts.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0712-1_24