Microwave-assisted functionalization of solid supports for rapid loading of nucleosides

• Published in: Current protocols in nucleic acid chemistry Vol. Chapter 3; p. Unit3.13
• Main Authors: Iyer, Radhakrishnan P, Padmanabhan, Seetharamaiyer, Coughlin, John E
• Format: Journal Article
• Language: English
• Published: United States 2006
• Subjects: Glass; Microwaves; Nucleosides - chemistry; Reproducibility of Results
• ISSN: 1934-9289
• DOI: 10.1002/0471142700.nc0313s23

Ultra-fast and efficient functionalization of solid supports such as controlled-pore glass (CPG), amino methyl polystyrene, and Tentagel has been achieved using microwave-assisted procedures. Both amino- and carboxy-terminated supports are easily prepared within minutes, in a reproducible manner, using microwave-assisted methodologies. The resulting functionalized supports are efficiently coupled to nucleosides using dimethylformamide as a solvent in conjunction with a specially designed reactor and workstation called LOTUS. Using these improved protocols, CPG with loadings of 75 to 85 micromol/g can be prepared on a large scale within 3 to 4 days starting from native CPG, as opposed to traditional methods that require 10 to 15 days to achieve the same objective. In addition, the methods described here can potentially be employed for rapid functionalization of other solid matrices such as beads, slides, and pins for applications in microarrays or combinatorial chemistry.