FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry

This chapter provides a protocol for a detailed evaluation of phytoplankton and nuisance cyanobacteria with the FlowCam 8400 and the FlowCam Cyano. The chapter includes (i) detailed description of the quality control of fluorescent mode of the FlowCam, (ii) detailing methods for discriminating nuisa...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2635; p. 219
Main Authors Roache-Johnson, Kathryn H, Stephens, Nicole R
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Chlorophyll
Cyanobacteria
Diagnostic Imaging
Flow Cytometry - methods
Phytoplankton
Staining and Labeling
Chlorophyll fluorescence
FlowCam
Freshwater cyanobacteria
Imaging flow cytometry
Classification
Phytoplankton
Viability staining
Phycocyanin fluorescence
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Summary:This chapter provides a protocol for a detailed evaluation of phytoplankton and nuisance cyanobacteria with the FlowCam 8400 and the FlowCam Cyano. The chapter includes (i) detailed description of the quality control of fluorescent mode of the FlowCam, (ii) detailing methods for discriminating nuisance cyanobacteria using the FlowCam Cyano, how to set up libraries and classification routines for commonly used classification reports, and (iii) detailing methods for viability staining to quantify LIVE versus DEAD phytoplankton using the FlowCam 8400.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3020-4_13