Extraction and SDS-PAGE Analysis of Proteins from Gualou Seeds

Protein extraction from Gualou seeds were studied, and Kjeldahl method and Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) were used to characterize the properties of extracted proteins. The optimum extraction parameters were as follows: the extraction solution was 0.2 mol/L ph...

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Bibliographic Details
Published inApplied Mechanics and Materials Vol. 644-650; pp. 5193 - 5196
Main Authors Yu, Jian, Zhao, Jing Bin, Li, Chi Lin, Liu, Yong Le
Format Journal Article
LanguageEnglish
Published Zurich Trans Tech Publications Ltd 01.09.2014
Subjects
SDS-PAGE
Extraction
Gualou seeds
Protein
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Summary:Protein extraction from Gualou seeds were studied, and Kjeldahl method and Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) were used to characterize the properties of extracted proteins. The optimum extraction parameters were as follows: the extraction solution was 0.2 mol/L phosphate buffer (pH, 8.0) with 5% NaCl, and the ratio of solid to liquid was 1:10. A protein yield of 75.01% was obtained under the optimal extraction conditions. The SDS-PAGE analysis showed that the proteins extracted from Gualou seeds have apparent molecular weights varying from 10 to 30 kDa, mainly close to 30 kDa.
Bibliography:Selected, peer reviewed papers from the 2014 International Conference on Machine Tool Technology and Mechatronics Engineering (ICMTTME 2014), June 22-23, 2014, Guilin, Guangxi, China
ISBN:9783038352464
3038352462
ISSN:1660-9336
1662-7482
1662-7482
DOI:10.4028/www.scientific.net/AMM.644-650.5193