Morphological and structural responsies of Fusobacterium nucleatum to fosfomycin
The effects of fosfomycin (FOM) on growth, morphology and the fine structure of Fusobacterium nucleatum cells were examined. The minimal inhibitory concentration (MIC) of FOM against F. nucleatum cells growing exponentially was 1.56μglml. Phase-contrast and scanning electron microscopy showed that t...
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| Published in | Japanese Journal of Oral Biology Vol. 27; no. 3; pp. 782 - 793 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Japanese Association for Oral Biology
1985
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0385-0137 |
| DOI | 10.2330/joralbiosci1965.27.782 |
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| Abstract | The effects of fosfomycin (FOM) on growth, morphology and the fine structure of Fusobacterium nucleatum cells were examined. The minimal inhibitory concentration (MIC) of FOM against F. nucleatum cells growing exponentially was 1.56μglml. Phase-contrast and scanning electron microscopy showed that the cells were elongated by exposure to 1/2 MIC of FOM, and vesicles were formed on the cells above the MIC. Transmission electron microscopy revealed that the vesicle was consisted of outer membrane only or outer membrane, cytoplasmic membrane and cytoplasm. Other changes in the cell structure than those mentioned above included invagination of outer membrane, cytoplasmic division and protoplast formation. It seems that the vesicle formation and the invagination of outer membrane are the structural changes related to cell lysis by FOM. |
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| AbstractList | The effects of fosfomycin (FOM) on growth, morphology and the fine structure of Fusobacterium nucleatum cells were examined. The minimal inhibitory concentration (MIC) of FOM against F. nucleatum cells growing exponentially was 1.56μglml. Phase-contrast and scanning electron microscopy showed that the cells were elongated by exposure to 1/2 MIC of FOM, and vesicles were formed on the cells above the MIC. Transmission electron microscopy revealed that the vesicle was consisted of outer membrane only or outer membrane, cytoplasmic membrane and cytoplasm. Other changes in the cell structure than those mentioned above included invagination of outer membrane, cytoplasmic division and protoplast formation. It seems that the vesicle formation and the invagination of outer membrane are the structural changes related to cell lysis by FOM. |
| Author | Konakajima, Yuichi Kaku, Nobuyuki Sagawa, Hirosuke Yoshitake, Hiroyuki Nakai, Yoshikazu Hirohata, Kenichi Onoe, Takatoshi |
| Author_xml | – sequence: 1 fullname: Kaku, Nobuyuki organization: Department of Microbiology, Osaka Dental University – sequence: 1 fullname: Nakai, Yoshikazu organization: Department of Microbiology, Osaka Dental University – sequence: 1 fullname: Konakajima, Yuichi organization: Department of Microbiology, Osaka Dental University – sequence: 1 fullname: Yoshitake, Hiroyuki organization: Department of Microbiology, Osaka Dental University – sequence: 1 fullname: Sagawa, Hirosuke organization: Department of Microbiology, Osaka Dental University – sequence: 1 fullname: Onoe, Takatoshi organization: Department of Microbiology, Osaka Dental University – sequence: 1 fullname: Hirohata, Kenichi organization: Department of Microbiology, Osaka Dental University |
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| References | 24) Spurr, A. R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastract Res. 26: 31, 1969. 9) 小中島諭一, 尾上孝利, 梅本俊夫, 谷明, 行徳友親, 河野忠雄, 佐川寛典: 抗生物質30種と化学療法剤4種に対するヒト口腔由来Fusobacteriumの感受性. Chemotherapy30: 1270-1277, 1982. 28) 中尾雅文, 近藤正煕, 土屋皖司: Cefmenoxime (SCE-1365) によるEscherichia coliおよびSerratia marcescensの形態学的変化について. Chemotherapy 29: 171-187, 1981. 15) 生方公子, 高橋洋子, 沢井稔, 斉藤洪太, 紺野昌俊: Cefmetazole (CS-1170) の抗菌作用に関する検討Chemotherapy 27: 652-667, 1979. 8) 佐村忠郎: ヒト口腔由来Lactobacilliの抗生物質感受性に関する研究.歯科医学45: 53-69, 1982. 32) Goodell, E. W., Fazio, M., Tomasz, A.: Effect of benzylpenicillin on the synthesis and structure of the cell envelope of Neisseria gonorrhoeae. Antimicrob Agents Chemother. 13: 514-526, 1978. 20) Blumberg, P. M., Strominger, J. L.: Interaction of penicillin with the bacterial cell: penicillin-binding protains and penicillin-sensitive enzymes. Bacteriol Rev. 38: 291-335, 1974. 22) 青沼清一, 大沼菊夫, 渡辺彰, 佐々木昌子, 大泉耕太郎, 今野淳: 抗生物質の併用に関する研究 (IV). Chemotherapy 30: 781-785, 1982. 1) Altucci, P., Abbate, G. F., Gattoni, A. Clinical evaluation of tobramycin in urinary tract infections. J Infect Dis. 134: S 139-141, 1976. 5) 応谷六郎: ヒト歯垢由来口腔レンサ球菌の抗生物質感受性に関する研究. 歯科医学46: 418-442, 1983. 23) Perea, E. J., Torres, M. A., Borobio, M. V.: Synergism of fosfomycin-ampicillin and fosfomycin-chloramphenicol against Salmonella and Shigella. Antimicrob Agents Chemother. 13: 705-709, 1978. 18) 宮内慶之輔, 吉田隆, 笠井隆夫, 斉藤喬子, 平野英子, 陶山佳代子, 吉村正子, 石井孝弘, 安部政弘, 鬼海庄一郎: Fosfomycinに関する細菌学的研究第1報. Jpn J Antibiot.28: 320-330, 1975. 25) Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 17: 208-211. 1963. 26) 尾上孝利, 杉中秀寿, 梅本俊夫, 小中島諭一, 佐川寛典: MecillinamによるFusobaeterium nucleatum細胞の伸長化. 日細菌誌36: 220, 1981. 21) 高橋公毅, 管野治重: 緑膿菌に対するピペラシリン・セフォペラゾン・セフスロジン・ホスホマイシン・トブラマイシン合併による併用効果. Chemotherapy 32: 966-971, 1984. 10) 中村長隆: ヒト口腔由来真菌の抗真菌剤感受性に関する研究. 歯科医学47: 1-22, 1984. 4) 駒井正, 浜田驍, 浜田泰三, 夕田貞之: 口腔カンジダ症の14例とカンジダ用簡易液体培地に関する研究, 日口外誌29: 893-898, 1983. 29) Klein, R., Luginbuhl, G. H.: Ampicillininduced morphological alterations of Haemophilus influenzae Type b. Antimicrob Agents Chemother. 11: 559-562, 1977. 6) 日高慧: 口腔より分離したレンサ球菌の抗生物質感受性に関する研究. 歯科医学41: 575-592, 1978. 11) 坂本邦基: 蛋白合成阻害抗生物質に対するStreptococcus mutans HS-6の感受性に関する電子顕微鏡学的研究, 歯科医学41: 544-574, 1978. 16) 杉浦朗, 武中一郎, 西野武志, 谷野輝雄: Bacteroides fragilisに対するβ-ラクタム系抗生物質作用時における形態変化とPBPの関連性について. Chemotherapy 30: 1509, 1982. 31) Nishino, T., Nakazawa, S.: Cephalexininduced morphological alterations in the surface structures of Staphylococcus aureus and Escherichia coli demonstrated by scanning electron microscopy. Jpn J Microbiol. 17: 383-392, 1973. 13) Onoe, T., Umemoto, T., Sagawa, H., Suginaka, H.: Filament formation of Fusobacterium nucleatum cells induced by mecillinam. Antimicrob Agents Chemother. 19: 487-489, 1981. 17) 二宮敬宇, 渡辺邦友, 甲畑俊郎, 分村博務, 三和敏夫, 上野一恵, 鈴木祥一郎, 望月泉, 清水保夫, 西浦常雄: Fosfbmycinの嫌気性菌に対する抗菌力. Chemotherapy 23: 1662-1668, 1975. 14) 中沢昭三, 西野武志, 山岸純一: 大腸菌に対するMecillinamの抗菌像. Chemotherapy25: 46-59, 1977. 3) 樋田謙二郎, 亀山嘉光, 野田邦治, 上原亀治, 高江州旭, 山田長敬: ブドウ糖非発酵グラム陰性桿菌感染による下顎骨骨髄炎の1例. 日口外誌30: 68-74, 1984. 12) 正井良夫: ペニシリン系およびセファロスポリン系抗生物質に対する齲蝕原性レンサ球菌の感受性に関する研究. 歯科医学41: 49-72, 1978. 19) Kahan, F. M., Kahan, J. S., Cassidy, P. J., Kropp, H.: The mechanism of action of fosfomycin. Ann NY Acad Sci. 235: 364-386, 1974. 30) Zimmerman, S. B., Stapley, E. O.: Relative morphological effects induced by cefoxitin and other beta-lactam antibiotics in vitro. Antimicrob Agents Chemother. 9: 318-326, 1976. 7) 倉松常俊: ヒト歯垢由来Actinomycesの抗生物質感受性に関する研究. 歯科医学45: 53-69, 1982. 27) Nakano, M., Nishi, T., Tsuchiya, K.: In vitro and in vivo morphological response of Klebsiella pneumoniae to cefotiam and cefazolin. Antimicrob. Agents Chemother. 19: 901-910, 1981. 2) 篠崎立彦, 小牧久美子, 柱新太郎, 藤井良和: 小児科領城におけるCefadroxilの投与成績Jpn J Antibiot. 34: 146-151, 1981. |
| References_xml | – reference: 9) 小中島諭一, 尾上孝利, 梅本俊夫, 谷明, 行徳友親, 河野忠雄, 佐川寛典: 抗生物質30種と化学療法剤4種に対するヒト口腔由来Fusobacteriumの感受性. Chemotherapy30: 1270-1277, 1982. – reference: 19) Kahan, F. M., Kahan, J. S., Cassidy, P. J., Kropp, H.: The mechanism of action of fosfomycin. Ann NY Acad Sci. 235: 364-386, 1974. – reference: 16) 杉浦朗, 武中一郎, 西野武志, 谷野輝雄: Bacteroides fragilisに対するβ-ラクタム系抗生物質作用時における形態変化とPBPの関連性について. Chemotherapy 30: 1509, 1982. – reference: 3) 樋田謙二郎, 亀山嘉光, 野田邦治, 上原亀治, 高江州旭, 山田長敬: ブドウ糖非発酵グラム陰性桿菌感染による下顎骨骨髄炎の1例. 日口外誌30: 68-74, 1984. – reference: 20) Blumberg, P. M., Strominger, J. L.: Interaction of penicillin with the bacterial cell: penicillin-binding protains and penicillin-sensitive enzymes. Bacteriol Rev. 38: 291-335, 1974. – reference: 2) 篠崎立彦, 小牧久美子, 柱新太郎, 藤井良和: 小児科領城におけるCefadroxilの投与成績Jpn J Antibiot. 34: 146-151, 1981. – reference: 28) 中尾雅文, 近藤正煕, 土屋皖司: Cefmenoxime (SCE-1365) によるEscherichia coliおよびSerratia marcescensの形態学的変化について. Chemotherapy 29: 171-187, 1981. – reference: 32) Goodell, E. W., Fazio, M., Tomasz, A.: Effect of benzylpenicillin on the synthesis and structure of the cell envelope of Neisseria gonorrhoeae. Antimicrob Agents Chemother. 13: 514-526, 1978. – reference: 15) 生方公子, 高橋洋子, 沢井稔, 斉藤洪太, 紺野昌俊: Cefmetazole (CS-1170) の抗菌作用に関する検討Chemotherapy 27: 652-667, 1979. – reference: 6) 日高慧: 口腔より分離したレンサ球菌の抗生物質感受性に関する研究. 歯科医学41: 575-592, 1978. – reference: 23) Perea, E. J., Torres, M. A., Borobio, M. V.: Synergism of fosfomycin-ampicillin and fosfomycin-chloramphenicol against Salmonella and Shigella. Antimicrob Agents Chemother. 13: 705-709, 1978. – reference: 12) 正井良夫: ペニシリン系およびセファロスポリン系抗生物質に対する齲蝕原性レンサ球菌の感受性に関する研究. 歯科医学41: 49-72, 1978. – reference: 10) 中村長隆: ヒト口腔由来真菌の抗真菌剤感受性に関する研究. 歯科医学47: 1-22, 1984. – reference: 4) 駒井正, 浜田驍, 浜田泰三, 夕田貞之: 口腔カンジダ症の14例とカンジダ用簡易液体培地に関する研究, 日口外誌29: 893-898, 1983. – reference: 31) Nishino, T., Nakazawa, S.: Cephalexininduced morphological alterations in the surface structures of Staphylococcus aureus and Escherichia coli demonstrated by scanning electron microscopy. Jpn J Microbiol. 17: 383-392, 1973. – reference: 24) Spurr, A. R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastract Res. 26: 31, 1969. – reference: 29) Klein, R., Luginbuhl, G. H.: Ampicillininduced morphological alterations of Haemophilus influenzae Type b. Antimicrob Agents Chemother. 11: 559-562, 1977. – reference: 22) 青沼清一, 大沼菊夫, 渡辺彰, 佐々木昌子, 大泉耕太郎, 今野淳: 抗生物質の併用に関する研究 (IV). Chemotherapy 30: 781-785, 1982. – reference: 5) 応谷六郎: ヒト歯垢由来口腔レンサ球菌の抗生物質感受性に関する研究. 歯科医学46: 418-442, 1983. – reference: 7) 倉松常俊: ヒト歯垢由来Actinomycesの抗生物質感受性に関する研究. 歯科医学45: 53-69, 1982. – reference: 17) 二宮敬宇, 渡辺邦友, 甲畑俊郎, 分村博務, 三和敏夫, 上野一恵, 鈴木祥一郎, 望月泉, 清水保夫, 西浦常雄: Fosfbmycinの嫌気性菌に対する抗菌力. Chemotherapy 23: 1662-1668, 1975. – reference: 14) 中沢昭三, 西野武志, 山岸純一: 大腸菌に対するMecillinamの抗菌像. Chemotherapy25: 46-59, 1977. – reference: 18) 宮内慶之輔, 吉田隆, 笠井隆夫, 斉藤喬子, 平野英子, 陶山佳代子, 吉村正子, 石井孝弘, 安部政弘, 鬼海庄一郎: Fosfomycinに関する細菌学的研究第1報. Jpn J Antibiot.28: 320-330, 1975. – reference: 21) 高橋公毅, 管野治重: 緑膿菌に対するピペラシリン・セフォペラゾン・セフスロジン・ホスホマイシン・トブラマイシン合併による併用効果. Chemotherapy 32: 966-971, 1984. – reference: 11) 坂本邦基: 蛋白合成阻害抗生物質に対するStreptococcus mutans HS-6の感受性に関する電子顕微鏡学的研究, 歯科医学41: 544-574, 1978. – reference: 13) Onoe, T., Umemoto, T., Sagawa, H., Suginaka, H.: Filament formation of Fusobacterium nucleatum cells induced by mecillinam. Antimicrob Agents Chemother. 19: 487-489, 1981. – reference: 8) 佐村忠郎: ヒト口腔由来Lactobacilliの抗生物質感受性に関する研究.歯科医学45: 53-69, 1982. – reference: 25) Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 17: 208-211. 1963. – reference: 27) Nakano, M., Nishi, T., Tsuchiya, K.: In vitro and in vivo morphological response of Klebsiella pneumoniae to cefotiam and cefazolin. Antimicrob. Agents Chemother. 19: 901-910, 1981. – reference: 1) Altucci, P., Abbate, G. F., Gattoni, A. Clinical evaluation of tobramycin in urinary tract infections. J Infect Dis. 134: S 139-141, 1976. – reference: 30) Zimmerman, S. B., Stapley, E. O.: Relative morphological effects induced by cefoxitin and other beta-lactam antibiotics in vitro. Antimicrob Agents Chemother. 9: 318-326, 1976. – reference: 26) 尾上孝利, 杉中秀寿, 梅本俊夫, 小中島諭一, 佐川寛典: MecillinamによるFusobaeterium nucleatum細胞の伸長化. 日細菌誌36: 220, 1981. |
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| Title | Morphological and structural responsies of Fusobacterium nucleatum to fosfomycin |
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