Methods and Technologies of Cholera Vibrio Cultivation (Scientific Review)

• Published in: Problemy osobo opasnyh infekcij no. 4; pp. 95 - 101
• Main Authors: Eremin, S. A., Aleshina, Yu. A., Komissarov, A. V., Gromova, O. V., Vasin, Yu. G., Nikiforov, A. K., Livanova, L. F., Volokh, O. A., Lobovikova, O. A., Bronnikova, V. S.
• Format: Journal Article
• Language: English; Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 20.12.2013
• Subjects: antigens; cultivation; technological parameters; vibrio choleraе; антигены; культивирование; технологические параметры
• ISSN: 0370-1069; 2658-719X
• DOI: 10.21055/0370-1069-2013-4-95-101

Displayed is the review of domestic and foreign literature sources devoted to matters of cholera vibrio cultivation. Discussed is the information concerning the following methods utilized in the technological process of vibrio growth stimulation: batch cultivation, fed-batch, fermentation and dialysis, periodic and continuous cultivation. Analyzed is the impact of such parameters as dissolved oxygen concentration, count of carbon nutrition source, medium pH, temperature rate , concentration and physiological condition of inoculate, duration of technological process, affecting the growth of this microorganism and synthesis of its antigens. Consequently, literature data analysis has contributed to the selection of proper method for cholera vibrio cultivation and consideration of the factors mentioned above for the development of manufacture technology applied to the production of medical immunoglobulin preparations for diagnostics and prophylaxis of cholera.