Quantification of Endocannabinoids from Biological Samples Using Liquid Chromatography-Tandem Mass Spectrometry

Endocannabinoids (eCBs) are a family of lipid molecules with important regulatory function in the brain and immune system. The two well-studied eCBs are arachidonic acid derivatives, N-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG). Currently one of the most important methods for q...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2625; p. 115
Main Authors Wang, Zhiying, Mo, Chenglin, Bonewald, Lynda, Brotto, Marco
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Arachidonic Acid
Brain
Chromatography, Liquid - methods
Endocannabinoids
Tandem Mass Spectrometry - methods
Brain
LC-MS/MS
Microdialysis
Endocannabinoids (eCBs)
Serum
Skeletal muscle
Solid-phase extraction
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Summary:Endocannabinoids (eCBs) are a family of lipid molecules with important regulatory function in the brain and immune system. The two well-studied eCBs are arachidonic acid derivatives, N-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG). Currently one of the most important methods for quantitative analysis of eCBs and related lipids from biological matrices is liquid chromatographic separation coupled with tandem mass spectroscopy (LC-MS/MS) owing to its high sensitivity and selectivity, as well as no derivatization procedures needed. Here we describe pretreatment procedures using solid-phase extraction for tissue sampling and an in vivo brain microdialysis approach prior to LC-MS/MS analysis, followed by detailed steps of LC-MS/MS analytic method to demonstrate the potential and application of this method in quantification of eCBs and congeners from various biological matrices.
ISSN:1940-6029
DOI:10.1007/978-1-0716-2966-6_11