Small angle X-ray scattering and 31P NMR studies on the phase behavior of phospholipid bilayered mixed micelles

The phase behavior of lipid bilayered micelles (`bicelles') (dimyristoyl-phosphatidylcholine, DMPC/dihexanoyl-phosphatidyl-choline, DHPC 2.6/1) has been studied by small angle X-ray scattering and 31P NMR. Below 3% w/v the bilayers are arranged in tightly packed stacks. At intermediate concentr...

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Published inChemical physics letters Vol. 329; no. 3; pp. 215 - 220
Main Authors Bolze, Jörg, Fujisawa, Tetsuro, Nagao, Takashi, Norisada, Kazushi, Saitô, Hazime, Naito, Akira
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.10.2000
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Summary:The phase behavior of lipid bilayered micelles (`bicelles') (dimyristoyl-phosphatidylcholine, DMPC/dihexanoyl-phosphatidyl-choline, DHPC 2.6/1) has been studied by small angle X-ray scattering and 31P NMR. Below 3% w/v the bilayers are arranged in tightly packed stacks. At intermediate concentrations single units are observed, whereas at 24% w/v and higher, weak stacking occurs again. The DMPC/DHPC ratio in the bicelles strongly increases at low concentration, which is correlated with an increase in the bicelle size and stacking. The increase of the order parameter in a magnetic field is related to the stack formation. Below 297 K there is no stacking at any concentration and no magnetic alignment.
ISSN:0009-2614
1873-4448
DOI:10.1016/S0009-2614(00)01029-0