Laser microdissection and genetic manipulation technologies to probe lignin heterogeneity and configuration in plant cell walls

Single and multiple T-DNA knockouts of genes encoding arogenate dehydratases (ADTs) in Arabidopsis were obtained in homozygous form. These were analyzed for potential differences in lignin contents and compositions, as well as for distinct phenotypes over growth and development. Of these different l...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 908; p. 229
Main Authors Corea, Oliver R A, Ki, Chanyoung, Cardenas, Claudia L, Davin, Laurence B, Lewis, Norman G
Format Journal Article
LanguageEnglish
Published United States 2012
Subjects
Arabidopsis - chemistry
Arabidopsis - enzymology
Arabidopsis - growth & development
DNA, Bacterial
Gas Chromatography-Mass Spectrometry
Gene Knockout Techniques - methods
Hydro-Lyases - deficiency
Hydro-Lyases - genetics
Laser Capture Microdissection - methods
Lignin - chemistry
Lignin - genetics
Molecular Conformation
Sequence Analysis, Protein
Spectrophotometry, Ultraviolet
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Summary:Single and multiple T-DNA knockouts of genes encoding arogenate dehydratases (ADTs) in Arabidopsis were obtained in homozygous form. These were analyzed for potential differences in lignin contents and compositions, as well as for distinct phenotypes over growth and development. Of these different lines, distinct reductions in lignin contents were obtained, with those having different G:S ratios depending upon the combination of ADT genes being knocked out. Results from pyrolysis GC/MS analyses indicated that differential carbon flux occurred into the vascular bundles (vb) and interfascicular fibers (if). These results provide additional new insight into factors controlling lignin heterogeneity and configuration.
ISSN:1940-6029
DOI:10.1007/978-1-61779-956-3_20