Anther Culture in Eggplant (Solanum melongena L.)

For a long time, conventional breeding methods have been used to obtain pure, 100% homozygous lines for hybrid seed production in crops of agronomic interest. However, by doubled haploid technology, it is possible to produce 100% homozygous plants derived from precursors of male gametophytes (androg...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 2122; p. 283
Main Authors Calabuig-Serna, Antonio, Porcel, Rosa, Corral-Martínez, Patricia, Seguí-Simarro, Jose M
Format Journal Article
LanguageEnglish
Published United States 2020
Subjects
DNA, Plant - genetics
Haploidy
Plant Breeding - methods
Plant Development
Ploidies
Pollen - genetics
Pollen - growth & development
Solanum melongena - genetics
Solanum melongena - growth & development
Tissue Culture Techniques - methods
Androgenesis
Tissue culture
Embryogenesis
Haploid
Doubled haploid
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Summary:For a long time, conventional breeding methods have been used to obtain pure, 100% homozygous lines for hybrid seed production in crops of agronomic interest. However, by doubled haploid technology, it is possible to produce 100% homozygous plants derived from precursors of male gametophytes (androgenesis), to accelerate the production of pure lines, which implies important time and cost savings. In this chapter, a protocol for anther culture in eggplant is described, from donor plant growth conditions to regeneration and acclimation of doubled haploid plants, as well as a description of how to analyze ploidy levels of regenerated plants.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0342-0_20