Quaternary structure of the radular muscle myglobin of the gastropod mollusc buccinum undatum L

1. 1. The radular muscle myoglobin of the gastropod mollusc Buccinum undatum has been purified. 2. 2. The myoglobin has a molecular weight of 33,800 and possesses two heme groups per molecule. 3. 3. When the myoglobin is reacted with excess p-hydroxymercuribenzoate (PMB), a single derivative of mole...

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Published inComparative biochemistry and physiology Vol. 31; no. 1; pp. 55 - 64
Main Authors Terwilliger, Robert C., Read, Kenneth R.H.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.10.1969
Subjects
Buccinum undatum
evolution of myoglobin
mollusc
Myoglobin— Buccinum
radular myoglobin
evolution of myoglobin
radular myoglobin
Myoglobin— Buccinum
Buccinum undatum
mollusc
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Summary:1. 1. The radular muscle myoglobin of the gastropod mollusc Buccinum undatum has been purified. 2. 2. The myoglobin has a molecular weight of 33,800 and possesses two heme groups per molecule. 3. 3. When the myoglobin is reacted with excess p-hydroxymercuribenzoate (PMB), a single derivative of molecular weight 16,900 is formed. 4. 4. The amino acid composition of the purified PMB derivative is: Lys 18His 7 Arg 2 Asp 16 Thr 14 Ser 8 Glu 5 Pro 2 Gly 13 Ala 19 1 2 Cys 2 Val 5 Met 5 Ileu 3 Leu 17 Tyr 1 Phy 10 Trp 3 . 5. 5. The native myoglobin is probably composed of two identical subunits. 6. 6. When the PMB-derivative is reacted successively with 2-mercaptoethanol and aminoiminoemethanesulfinic acid, a dimeric myoglobin is regenerated. 7. 7. The evolutionary relationship of Buccinum myoglobin to other gastropod myoglobins is discussed.
ISSN:0010-406X
DOI:10.1016/0010-406X(69)92168-9