Improvements in microphotometry by digital signal processing

From noise measurements with a microscope photometer it is deduced that the square roots of the measured intensities are linear with respect to photon noise. It is demonstrated by using a cytological specimen with a large dynamic range of intensities, that an IIR filter uniformly smoothes the square...

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Bibliographic Details
Published inPattern recognition Vol. 13; no. 1; pp. 79 - 82
Main Authors Zimmer, Hans-Georg, Kronberg, Harald, Bernstein, Reinhard, Neuhoff, Volker
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 1981
Subjects
Cytophotometry
Digital image processing
Linear filtering
Noise reduction
Signal models
Signal models
Linear filtering
Noise reduction
Cytophotometry
Digital image processing
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Summary:From noise measurements with a microscope photometer it is deduced that the square roots of the measured intensities are linear with respect to photon noise. It is demonstrated by using a cytological specimen with a large dynamic range of intensities, that an IIR filter uniformly smoothes the square roots of the intensities, whereas the effect of that filter on the measured intensities is larger in dark parts of the image than in bright areas.
ISSN:0031-3203
1873-5142
DOI:10.1016/0031-3203(81)90034-0