Plasminogen activator inhibitor induced by lipopolysaccharide injection in the rat Zymographic analysis

• Published in: Fibrinolysis Vol. 1; no. 1; pp. 45 - 49
• Main Authors: Dosne, A.M., Dubor, F., Chedid, L.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 1987
• Subjects: Lipopolysaccharide; Plasminogen activator inhibitor; Polymyxin; Lipopolysaccharide; Polymyxin; Plasminogen activator inhibitor
• ISSN: 0268-9499
• DOI: 10.1016/0268-9499(87)90032-4

It has been shown that endotoxin injection induces formation of Plasminogen Activator Inhibitor (PAI). The present study shows that a decrease in euglobulin fibrinolytic activity 4 h after lipopolysaccharide (LPS) injection is associated with an increase in urokinase neutralisation, either by euglobulin or plasma. After 30 μg/kg LPS this inhibitor reaches 6 U/ml in euglobulin and 41 U/ml in plasma. Zymographic analysis of post LPS euglobulin shows evidence of an increased PAI resulting in a reduction of tissue plasminogen activator (tPA) ( M r 68 000) and formation of higher complexes ( M r, 105 000), but development time is critical for detecting these modifications simultaneously. Increase in a urokinase binding factor in post-LPS plasma or euglobulin is clearly demonstrated from reduction in urokinase lytic bands ( M r, 34 000 and 54 000) with a parallel generation of a large complex ( M r 80 000 to 105 000). From this shift, the mol. weight of PAI may be estimated to be 46 000–51000. Combination of Polymyxin B or Colimycin with LPS could prevent the decrease in euglobulin fibrinolytic activity in low endotoxemia.