Hemoglobin and myoglobin embedded in dry poly(vinyl alcohol) film for X-ray absorption studies

Solid hemoprotein samples are prepared by embedding proteins in thin poly(vinyl alcohol) films. These film samples have several unique qualities: (1) in the solid poly(vinyl alcohol) matrix, hemoproteins have the same properties as in frozen buffer solution (proven by optical absorption, ligand reco...

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Bibliographic Details
Published inBiochimica et biophysica acta, Protein structure and molecular enzymology Vol. 874; no. 1; pp. 13 - 18
Main Authors Teng, Tsu-Yi, Huang, Huey W.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 07.11.1986
Subjects
Hemoglobin
Myoglobin
Poly(vinyl alcohol) film
Solid hemoprotein sample
Time-resolved EXAFS
Myoglobin
Solid hemoprotein sample
Hepes
Hemoglobin
Poly(vinyl alcohol) film
Time-resolved EXAFS
EXAFS
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Summary:Solid hemoprotein samples are prepared by embedding proteins in thin poly(vinyl alcohol) films. These film samples have several unique qualities: (1) in the solid poly(vinyl alcohol) matrix, hemoproteins have the same properties as in frozen buffer solution (proven by optical absorption, ligand recombination kinetics and EXAFS); (2) they are very stable, easy to store and resistant to radiation damage; (3) protein concentration can be as high as 15mM; (4) light transparency is as good as liquid solution samples; and (5) they can be made as thin as 20 μm, so that 100% photolysis across a film, even with a high protein concentration, is easily achievable. The film samples are ideal for X-ray studies of optically illuminated hemoproteins.
ISSN:0167-4838
1879-2588
DOI:10.1016/0167-4838(86)90095-6