Microsomal chemiluminescence of benzo[ a]pyrene-7,8-dihydrodiol and its synthetic analogues trans- and cis-1-methoxyvinylpyrene
The cis- and trans-methoxyvinylpyrene (MVP) analogues of benzo[ a]pyrene-7,8-dihydrodiol (7,8-diol) produce specific microsomal chemiluminescence comparable to that produced from 7,8-diol in Aroclor-induced rat liver microsome preparations. The chemiluminescence quantum yields, emission spectra, and...
Saved in:
Published in | Biochimica et biophysica acta. General subjects Vol. 882; no. 2; pp. 210 - 219 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
19.06.1986
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The
cis- and
trans-methoxyvinylpyrene (MVP) analogues of benzo[
a]pyrene-7,8-dihydrodiol (7,8-diol) produce specific microsomal chemiluminescence comparable to that produced from 7,8-diol in Aroclor-induced rat liver microsome preparations. The chemiluminescence quantum yields, emission spectra, and the concentration and the temporal kinetics of these three substrates have been examined. Radiolabelled 7,8-diol and
t-MVP exhibit significant covalent binding (more than 14%) to microsomal protein when metabolized enzymatically. The extreme quenching of the dioxetane chemiluminescence by both microsomes and phosphatidylcholine, as a model phospholipid, implies that despite the low quantum yield (approx. 10
−8 photons per substrate molecule) for microsomal chemiluminescence of these substrates, a significant fraction of their microsomal oxygenations may proceed via a dioxetane pathway. |
---|---|
ISSN: | 0304-4165 1872-8006 |
DOI: | 10.1016/0304-4165(86)90157-1 |