Human T-cell proliferation following PPD presentation by monocytes: Inhibition by anti- β2-microglobulin

• Published in: Clinical immunology and immunopathology Vol. 15; no. 2; pp. 123 - 132
• Main Authors: McCalmon, R.T., Kirkegaard, D.A., Kubo, R.T., Grey, H.M.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 1980
• ISSN: 0090-1229; 1090-2341
• DOI: 10.1016/0090-1229(80)90025-2

Peripheral blood lymphocytes were obtained from PPD-sensitive individuals and separated into a T-lymphocyte-enriched population and a monocyte-enriched population. The monocyte population was treated with mitomycin C, then pulsed with PPD. When the mitomycin C-treated, PPD-pulsed cells were cocultured with autologous T lymphocytes, the T cells proliferated demonstrating that human monocytes present antigen to T lymphocytes. When mitomycin C-treated, antigen-pulsed monocytes were preincubated with anti- β 2m, no effect on the proliferative response was observed. However, when the responding T cells were preincubated with anti- β 2m, the proliferative response was inhibited. Similarly, the effect of anti-HLA and anti-Ia on the PPD-induced proliferative response was studied. The results obtained demonstrated that anti-HLA had little or no effect on the proliferative response while anti-Ia appeared to inhibit the responding lymphocytes to the same extent as the antigen-pulsed stimulating cells.