The solubilization of an elastin-like protein from copper-deficient porcine aorta

1. 1. A soluble elastin-like protein is present in the aortas of copper-deficient swine. Studies were undertaken to determine the optimal means of selectively solubilizing this protein. 2. 2. Swelling and solubilization studies on copper-deficient and normal, young, porcine aortas over a range of pH...

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Published inBiochimica et biophysica acta. Protein structure Vol. 181; no. 1; pp. 201 - 207
Main Authors Sandberg, L.B., Hackett, T.N., Carnes, W.H.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 20.05.1969
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Summary:1. 1. A soluble elastin-like protein is present in the aortas of copper-deficient swine. Studies were undertaken to determine the optimal means of selectively solubilizing this protein. 2. 2. Swelling and solubilization studies on copper-deficient and normal, young, porcine aortas over a range of pH values revealed a marked difference in the behavior of the two tissues. Sharp maxima of swelling and solubilization were observed in the copper-deficient tissue at pH 3 and 7. 3. 3. By further studies of the effects of salt at these pH's, it was determined that a relatively pure elastin-like protein can be solubilized from the copper-deficient aorta at pH 3 and low salt concentration. A method of extracting the aorta under these conditions is described.
ISSN:0005-2795
1879-2952
DOI:10.1016/0005-2795(69)90242-6