Quantitative imaging of atomic and molecular species in cancer cell cultures with TOF-SIMS and Laser-SNMS

For boron neutron capture therapy, a promising cancer therapy under development, knowledge about the subcellular boron distribution is important. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) and nonresonant laser secondary neutral mass spectrometry (NR-Laser-SNMS) have been used for exa...

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Bibliographic Details
Published inApplied surface science Vol. 231; pp. 428 - 431
Main Authors Fartmann, M., Kriegeskotte, C., Dambach, S., Wittig, A., Sauerwein, W., Arlinghaus, H.F.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.06.2004
Subjects
BNCT
Boron
Cell
Imaging
Laser-SNMS
TOF-SIMS
Laser-SNMS
BNCT
Boron
TOF-SIMS
Cell
Imaging
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Summary:For boron neutron capture therapy, a promising cancer therapy under development, knowledge about the subcellular boron distribution is important. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) and nonresonant laser secondary neutral mass spectrometry (NR-Laser-SNMS) have been used for examining freeze-fractured, freeze-dried human melanoma cells incubated with sodium mercaptoundecahydro-closo-dodecaborate ( Na 2 10 B 12 H 11 SH, BSH ), a 10 B containing drug. With both techniques, elemental and molecular images were obtained from the cancer cells with very high sensitivity and subcellular resolution. The measurement of the K/Na ratio demonstrated that the preparation technique used was appropriate for preserving the chemical and structural integrity of living cells. The boron images showed that the intensity of intracellular and extracellular boron signals was clearly different after incubation of cells in different boron concentrations.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2004.03.160