In Vivo Monitoring of Cytosolic pH Using the Ratiometric pH Sensor pHluorin

Cytosolic pH (pH ) is a key factor controlling cell fate. The genetically encoded pH-sensor pHluorin has proven highly valuable for studies on pH in many living organisms. pHluorin displays a bimodal excitation spectrum with peaks at 395 nm and 475 nm, which is dependent on pH. Here we describe two...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2391; p. 99
Main Authors Fernandes, Tânia R, Serrano, Antonio, Di Pietro, Antonio
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Cytosol
Fusarium
Green Fluorescent Proteins
Hydrogen-Ion Concentration
Spectrofluorometer
Ratiometric pH sensor
Cytosolic pH
Confocal microscope
pHluorin
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Summary:Cytosolic pH (pH ) is a key factor controlling cell fate. The genetically encoded pH-sensor pHluorin has proven highly valuable for studies on pH in many living organisms. pHluorin displays a bimodal excitation spectrum with peaks at 395 nm and 475 nm, which is dependent on pH. Here we describe two different protocols for determining pH in the soil-borne fungal pathogen Fusarium oxysporum, based either on population or single-cell analysis.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1795-3_9