Comparative analysis of the pig BAC sequence involved in the regulation of myostatin gene

• Published in: Science China. Life sciences Vol. 48; no. 2; pp. 168 - 180
• Main Authors: Yu, Zhengquan, Li, Yan, Meng, Qingyong, Yuan, Jing, Zhao, Zhihui, Li, Wei, Hu, Xiaoxiang, Yan, Bingxue, Fan, Baoliang, Yu, Shuyang, Li, Ning
• Format: Journal Article
• Language: English
• Published: China Springer Nature B.V 01.09.2005; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China%Beijing Genomics Institute / Genomics and Bioinformatics Center, Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, China
• Subjects: 3' Flanking Region; 3' Untranslated Regions; Animals; Bacterial artificial chromosomes; Base Sequence; Binding Sites; Chromosomes, Artificial, Bacterial - chemistry; Cloning, Molecular; Comparative analysis; Conserved Sequence; CpG Islands; Evolution, Molecular; Evolutionary conservation; Exons; Gene Expression Regulation, Developmental; Genome, Human; Humans; Mice; Molecular Sequence Data; Myostatin; Polyadenylation; Promoter Regions, Genetic; Sequence Analysis, DNA; Skeletal muscle; Swine; Tandem Repeat Sequences; TATA Box; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transforming Growth Factor beta - genetics; Transforming growth factor-b; comparative analysis; transcriptional factors; muscle development; myostatin
• ISSN: 1006-9305; 1674-7305; 1862-2798; 1869-1889
• DOI: 10.1007/BF02879670

Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass. In order to study its expression and regulatory mechanism deeply, we have presented a comparative analysis of about 170-kb pig BAC sequence containing the myostatin gene among pig, human and mouse. The genomic region is characterized by high interspersed repeats and low G+C content. As for the myostatin gene, a higher sequence similarity is found between human and pig than between these species and the mouse. One striking feature is that the structure of two TATA-boxes in the nearby downstream of CCAAT-box is identified in the promoter. Further analysis reveals that the TATA-box1 is responsible for the transcription in pig and human, but the TATA-box2 acts on the transcription in mouse. The other interesting feature is that two polyadenylation signal sequences (AATAAA) exist in 3'UTR of the pig myostatin gene. Moreover, a large number of potential transcription factor-binding sites are also identified in evolutionary conserved regions (ECRs), which may be associated with the regulation of myostatin. Many putative transcription factors play an important role in the muscle development, and the complex interaction between myostatin and these factors may be required for proper muscle development.