Efficient and Fast Production of Transgenic Rice Plants by Agrobacterium-Mediated Transformation

Genetic transformation plays a key role in deciphering regulation of agronomic traits at molecular level in rice, a model monocot cereal crop. Here we describe an efficient and fast protocol for producing transgenic japonica rice plants using the Agrobacterium-mediated transformation method. The pro...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1864; p. 95
Main Authors Wu, Chuanyin, Sui, Yi
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Agrobacterium - genetics
Oryza - genetics
Plant Shoots
Plants, Genetically Modified - genetics
Seeds
Time Factors
Tissue Culture Techniques - instrumentation
Tissue Culture Techniques - methods
Transformation, Genetic
Callus induction
Agrobacterium-mediated transformation
Plant regeneration
Selection of transformants
Rice
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Summary:Genetic transformation plays a key role in deciphering regulation of agronomic traits at molecular level in rice, a model monocot cereal crop. Here we describe an efficient and fast protocol for producing transgenic japonica rice plants using the Agrobacterium-mediated transformation method. The protocol simplifies medium compositions and transformation steps and can be easily followed by a lab technician with little tissue culture experience. Using this protocol, we have transformed thousands of gene constructs in the past 10 years and edited hundreds of genes with the CRISPR-Cas9 system recently.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8778-8_7